1mdw

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[[Image:1mdw.gif|left|200px]]
 
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==Crystal Structure of Calcium-Bound Protease Core of Calpain II Reveals the Basis for Intrinsic Inactivation==
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The line below this paragraph, containing "STRUCTURE_1mdw", creates the "Structure Box" on the page.
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<StructureSection load='1mdw' size='340' side='right'caption='[[1mdw]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1mdw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MDW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MDW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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{{STRUCTURE_1mdw| PDB=1mdw | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mdw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mdw OCA], [https://pdbe.org/1mdw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mdw RCSB], [https://www.ebi.ac.uk/pdbsum/1mdw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mdw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CAN2_RAT CAN2_RAT] Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/md/1mdw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mdw ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of Calcium-Bound Protease Core of Calpain II Reveals the Basis for Intrinsic Inactivation'''
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==See Also==
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*[[Calpain 3D structures|Calpain 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Uncontrolled activation of calpain can lead to necrotic cell death and irreversible tissue damage. We have discovered an intrinsic mechanism whereby the autolysis-generated protease core fragment of calpain is inactivated through the inherent instability of a key alpha-helix. This auto-inactivation state was captured by the 1.9 A Ca(2+)-bound structure of the protease core from m-calpain, and sequence alignments suggest that it applies to about half of the calpain isoforms. Intact calpain large subunits are also subject to this inhibition, which can be prevented through assembly of the heterodimers. Other isoforms or their released cores are not silenced by this mechanism and might contribute to calpain patho-physiologies.
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[[Category: Large Structures]]
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==About this Structure==
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1MDW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MDW OCA].
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==Reference==
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Calpain silencing by a reversible intrinsic mechanism., Moldoveanu T, Hosfield CM, Lim D, Jia Z, Davies PL, Nat Struct Biol. 2003 May;10(5):371-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12665854 12665854]
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[[Category: Hydrolase]]
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Davies PL]]
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[[Category: Davies, P L.]]
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[[Category: Hosfield CM]]
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[[Category: Hosfield, C M.]]
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[[Category: Jia Z]]
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[[Category: Jia, Z.]]
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[[Category: Lim D]]
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[[Category: Lim, D.]]
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[[Category: Moldoveanu T]]
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[[Category: Moldoveanu, T.]]
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[[Category: Calpain cysteine protease fold]]
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[[Category: Helix instability]]
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[[Category: Tryptophan-based active site blockage]]
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[[Category: Two cooperative calcium site]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:55:33 2008''
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Current revision

Crystal Structure of Calcium-Bound Protease Core of Calpain II Reveals the Basis for Intrinsic Inactivation

PDB ID 1mdw

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