1mey

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[[Image:1mey.gif|left|200px]]
 
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==CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA==
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The line below this paragraph, containing "STRUCTURE_1mey", creates the "Structure Box" on the page.
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<StructureSection load='1mey' size='340' side='right'caption='[[1mey]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1mey]] is a 7 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MEY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MEY FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C38:5-IODO-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>C38</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1mey| PDB=1mey | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mey FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mey OCA], [https://pdbe.org/1mey PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mey RCSB], [https://www.ebi.ac.uk/pdbsum/1mey PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mey ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA'''
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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==Overview==
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<jmolCheckbox>
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Considerable recent effort has been devoted to the design and selection of sequence-specific DNA binding proteins based on tandem arrays of Cys2His2 zinc finger domains. While the DNA binding properties of these designed proteins have been studied extensively, the structural basis for site-specific binding has not been examined experimentally. Here we report the crystal structure of a complex between a protein comprised of three consensus-sequence-based zinc finger domains and an oligonucleotide corresponding to a favourable DNA binding site. This structure reveals relatively simple modular interactions and structural adaptations that compensate for differences in contact residue side-chain lengths.
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/me/1mey_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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==About this Structure==
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<text>to colour the structure by Evolutionary Conservation</text>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MEY OCA].
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mey ConSurf].
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==Reference==
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<div style="clear:both"></div>
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A 2.2 A resolution crystal structure of a designed zinc finger protein bound to DNA., Kim CA, Berg JM, Nat Struct Biol. 1996 Nov;3(11):940-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8901872 8901872]
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__TOC__
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[[Category: Berg, J M.]]
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</StructureSection>
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[[Category: Kim, C A.]]
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[[Category: Large Structures]]
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[[Category: Crystal structure]]
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[[Category: Berg JM]]
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[[Category: Protein design]]
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[[Category: Kim CA]]
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[[Category: Protein-dna interaction]]
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[[Category: Zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:57:25 2008''
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Current revision

CRYSTAL STRUCTURE OF A DESIGNED ZINC FINGER PROTEIN BOUND TO DNA

PDB ID 1mey

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