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| - | [[Image:1mf5.gif|left|200px]] | |
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| - | <!--
| + | ==GCATGCT Quadruplex== |
| - | The line below this paragraph, containing "STRUCTURE_1mf5", creates the "Structure Box" on the page.
| + | <StructureSection load='1mf5' size='340' side='right'caption='[[1mf5]], [[Resolution|resolution]] 1.10Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1mf5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MF5 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1Å</td></tr> |
| - | -->
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr> |
| - | {{STRUCTURE_1mf5| PDB=1mf5 | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mf5 OCA], [https://pdbe.org/1mf5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mf5 RCSB], [https://www.ebi.ac.uk/pdbsum/1mf5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mf5 ProSAT]</span></td></tr> |
| - | | + | </table> |
| - | '''GCATGCT Quadruplex''' | + | __TOC__ |
| - | | + | </StructureSection> |
| - | | + | [[Category: Large Structures]] |
| - | ==Overview== | + | [[Category: Cardin CJ]] |
| - | Here we report the crystal structure of the DNA heptanucleotide sequence d(GCATGCT) determined to a resolution of 1.1 A. The sequence folds into a complementary loop structure generating several unusual base pairings and is stabilised through cobalt hexammine and highly defined water sites. The single stranded loop is bound together through the G(N2)-C(O2) intra-strand H-bonds for the available G/C residues, which form further Watson-Crick pairings to a complementary sequence, through 2-fold symmetry, generating a pair of non-planar quadruplexes at the heart of the structure. Further, four adenine residues stack in pairs at one end, H-bonding through their N7-N6 positions, and are additionally stabilised through two highly conserved water positions at the structural terminus. This conformation is achieved through the rotation of the central thymine base at the pinnacle of the loop structure, where it stacks with an adjacent thymine residue within the lattice. The crystal packing yields two halved biological units, each related across a 2-fold symmetry axis spanning a cobalt hexammine residue between them, which stabilises the quadruplex structure through H-bonds to the phosphate oxygens and localised hydration.
| + | [[Category: Gale BC]] |
| - | | + | [[Category: Teixeira SCM]] |
| - | ==About this Structure==
| + | [[Category: Thorpe JH]] |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MF5 OCA]. | + | |
| - | | + | |
| - | ==Reference== | + | |
| - | Crystal structure of the complementary quadruplex formed by d(GCATGCT) at atomic resolution., Thorpe JH, Teixeira SC, Gale BC, Cardin CJ, Nucleic Acids Res. 2003 Feb 1;31(3):844-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12560479 12560479]
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| - | [[Category: Cardin, C J.]] | + | |
| - | [[Category: Gale, B C.]] | + | |
| - | [[Category: Teixeira, S C.M.]] | + | |
| - | [[Category: Thorpe, J H.]] | + | |
| - | [[Category: Atomic resolution]] | + | |
| - | [[Category: Quadruplex]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:57:42 2008''
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