1mju

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[[Image:1mju.gif|left|200px]]
 
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==1.22 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF THE FAB FRAGMENT OF ESTEROLYTIC ANTIBODY MS6-12==
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The line below this paragraph, containing "STRUCTURE_1mju", creates the "Structure Box" on the page.
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<StructureSection load='1mju' size='340' side='right'caption='[[1mju]], [[Resolution|resolution]] 1.22&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1mju]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MJU FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.22&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
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{{STRUCTURE_1mju| PDB=1mju | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mju OCA], [https://pdbe.org/1mju PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mju RCSB], [https://www.ebi.ac.uk/pdbsum/1mju PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mju ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mj/1mju_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mju ConSurf].
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<div style="clear:both"></div>
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'''1.22 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF THE FAB FRAGMENT OF ESTEROLYTIC ANTIBODY MS6-12'''
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==See Also==
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The crystal structures of four related Fab fragments of a family of catalytic antibodies displaying differential levels of esterase activity have been solved in the presence and in the absence of the transition-state analogue (TSA) that was used to elicit the immune response. The electron density maps show that the TSA conformation is essentially identical, with limited changes on hapten binding. Interactions with the TSA explain the specificity for the D rather than the L-isomer of the substrate. Differences in the residues in the hapten-binding pocket, which increase hydrophobicity, appear to correlate with an increase in the affinity of the antibodies for their substrate. Analysis of the structures at the active site reveals a network of conserved hydrogen bond contacts between the TSA and the antibodies, and points to a critical role of two conserved residues, HisL91 and LysH95, in catalysis. However, these two key residues are set into very different contexts in their respective structures, with an apparent direct correlation between the catalytic power of the antibodies and the complexity of their interactions with the rest of the protein. This suggests that the catalytic efficiency may be controlled by contacts arising from a second sphere of residues at the periphery of the active site.
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[[Category: Large Structures]]
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[[Category: Mus musculus]]
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==About this Structure==
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[[Category: Blackburn GM]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MJU OCA].
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[[Category: Kakinuma H]]
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[[Category: Muranova TA]]
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==Reference==
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[[Category: Murray IA]]
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High-resolution crystal structure of the Fab-fragments of a family of mouse catalytic antibodies with esterase activity., Ruzheinikov SN, Muranova TA, Sedelnikova SE, Partridge LJ, Blackburn GM, Murray IA, Kakinuma H, Takahashi-Ando N, Shimazaki K, Sun J, Nishi Y, Rice DW, J Mol Biol. 2003 Sep 12;332(2):423-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12948492 12948492]
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[[Category: Nishi Y]]
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[[Category: Blackburn, G M.]]
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[[Category: Partridge LJ]]
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[[Category: Kakinuma, H.]]
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[[Category: Rice DW]]
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[[Category: Muranova, T A.]]
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[[Category: Ruzheinikov SN]]
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[[Category: Murray, I A.]]
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[[Category: Sedelnikova SE]]
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[[Category: Nishi, Y.]]
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[[Category: Shimazaki K]]
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[[Category: Partridge, L J.]]
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[[Category: Sun J]]
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[[Category: Rice, D W.]]
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[[Category: Takashi N]]
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[[Category: Ruzheinikov, S N.]]
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[[Category: Sedelnikova, S E.]]
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[[Category: Shimazaki, K.]]
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[[Category: Sun, J.]]
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[[Category: Takashi, N.]]
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[[Category: Catalytic antibody]]
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[[Category: Ester hydrolysis]]
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[[Category: Esterolytic]]
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[[Category: Fab]]
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[[Category: Immunoglobulin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:13:56 2008''
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Current revision

1.22 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF THE FAB FRAGMENT OF ESTEROLYTIC ANTIBODY MS6-12

PDB ID 1mju

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