1mlx

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:45, 14 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1mlx.gif|left|200px]]
 
-
<!--
+
==Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex==
-
The line below this paragraph, containing "STRUCTURE_1mlx", creates the "Structure Box" on the page.
+
<StructureSection load='1mlx' size='340' side='right'caption='[[1mlx]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1mlx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MLX FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SMT:2-[(METHYLTHIO)ETHYLOXY]-THYMIDINE-5-MONOPHOSPHATE'>SMT</scene></td></tr>
-
{{STRUCTURE_1mlx| PDB=1mlx | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mlx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mlx OCA], [https://pdbe.org/1mlx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mlx RCSB], [https://www.ebi.ac.uk/pdbsum/1mlx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mlx ProSAT]</span></td></tr>
-
 
+
</table>
-
'''Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex'''
+
__TOC__
-
 
+
</StructureSection>
-
 
+
[[Category: Large Structures]]
-
==Overview==
+
[[Category: Egli M]]
-
A novel 2'-modification, 2'-O-[2-(methylthio)ethyl] or 2'-O-MTE, has been incorporated into oligonucleotides and evaluated for properties relevant to antisense activity. The results were compared with the previously characterized 2'-O-[2-(methoxy)ethyl] 2'-O-MOE modification. As expected, the 2'-O-MTE modified oligonucleotides exhibited improved binding to human serum albumin compared to the 2'-O-MOE modified oligonucleotides. The 2'-O-MTE oligonucleotides maintained high binding affinity to target RNA. Nuclease digestion of 2'-O-MTE oligonucleotides showed that they have limited resistance to exonuclease degradation. We analyzed the crystal structure of a decamer DNA duplex containing the 2'-O-MTE modifcation. Analysis of the crystal structure provides insight into the improved RNA binding affinity, protein binding affinity and limited resistance of 2'-O-MTE modified oligonucleotides to exonuclease degradation.
+
[[Category: Fraser AS]]
-
 
+
[[Category: Kawasaki AM]]
-
==About this Structure==
+
[[Category: Leeds JM]]
-
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MLX OCA].
+
[[Category: Lesnik EA]]
-
 
+
[[Category: Manoharan M]]
-
==Reference==
+
[[Category: Prakash TP]]
-
2'-O-[2-(methylthio)ethyl]-modified oligonucleotide: an analogue of 2'-O-[2-(methoxy)-ethyl]-modified oligonucleotide with improved protein binding properties and high binding affinity to target RNA., Prakash TP, Manoharan M, Kawasaki AM, Fraser AS, Lesnik EA, Sioufi N, Leeds JM, Teplova M, Egli M, Biochemistry. 2002 Oct 1;41(39):11642-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12269806 12269806]
+
[[Category: Sioufi N]]
-
[[Category: Egli, M.]]
+
[[Category: Teplova M]]
-
[[Category: Fraser, A S.]]
+
-
[[Category: Kawasaki, A M.]]
+
-
[[Category: Leeds, J M.]]
+
-
[[Category: Lesnik, E A.]]
+
-
[[Category: Manoharan, M.]]
+
-
[[Category: Prakash, T P.]]
+
-
[[Category: Sioufi, N.]]
+
-
[[Category: Teplova, M.]]
+
-
[[Category: A-form]]
+
-
[[Category: Antisense modification]]
+
-
[[Category: Dna]]
+
-
[[Category: Double helix]]
+
-
[[Category: High resolution structure]]
+
-
[[Category: Hydration]]
+
-
[[Category: Nuclease resistance]]
+
-
[[Category: Nucleic acid analogue]]
+
-
[[Category: Protein binding affinity]]
+
-
[[Category: Rna affinity]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:23:51 2008''
+

Current revision

Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex

PDB ID 1mlx

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1mlx"
Personal tools