1mx3

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(New page: 200px<br /> <applet load="1mx3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mx3, resolution 1.95&Aring;" /> '''Crystal structure o...)
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[[Image:1mx3.gif|left|200px]]<br />
 
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<applet load="1mx3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1mx3, resolution 1.95&Aring;" />
 
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'''Crystal structure of CtBP dehydrogenase core holo form'''<br />
 
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==Overview==
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==Crystal structure of CtBP dehydrogenase core holo form==
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Transcriptional repression is based on the selective actions of recruited, corepressor complexes, including those with enzymatic activities. One, well-characterized developmentally important corepressor is the C-terminal, binding protein (CtBP). Although intriguingly related in sequence to D2, hydroxyacid dehydrogenases, the mechanism by which CtBP functions remains, unclear. We report here biochemical and crystallographic studies which, reveal that CtBP is a functional dehydrogenase. In addition, both a, cofactor-dependent conformational change, with NAD(+) and NADH being, equivalently effective, and the active site residues are linked to the, binding of the PXDLS consensus recognition motif on repressors, such as, E1A and RIP140. Together, our data suggest that CtBP is an, NAD(+)-regulated component of critical complexes for specific repression, events in cells.
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<StructureSection load='1mx3' size='340' side='right'caption='[[1mx3]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1mx3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MX3 FirstGlance]. <br>
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1MX3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAD and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1MX3 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mx3 OCA], [https://pdbe.org/1mx3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mx3 RCSB], [https://www.ebi.ac.uk/pdbsum/1mx3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mx3 ProSAT]</span></td></tr>
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Transcription corepressor CtBP is an NAD(+)-regulated dehydrogenase., Kumar V, Carlson JE, Ohgi KA, Edwards TA, Rose DW, Escalante CR, Rosenfeld MG, Aggarwal AK, Mol Cell. 2002 Oct;10(4):857-69. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12419229 12419229]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CTBP1_HUMAN CTBP1_HUMAN] Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.<ref>PMID:9858600</ref> <ref>PMID:15542832</ref> <ref>PMID:19103759</ref> <ref>PMID:12419229</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mx/1mx3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mx3 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Aggarwal, A.K.]]
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[[Category: Aggarwal AK]]
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[[Category: Carlson, J.E.]]
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[[Category: Carlson JE]]
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[[Category: Edwards, T.E.]]
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[[Category: Edwards TE]]
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[[Category: Escalante, C.R.]]
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[[Category: Escalante CR]]
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[[Category: Kumar, V.]]
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[[Category: Kumar V]]
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[[Category: Ohgi, K.E.]]
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[[Category: Ohgi KE]]
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[[Category: Rose, D.W.]]
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[[Category: Rose DW]]
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[[Category: ACY]]
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[[Category: NAD]]
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[[Category: nuclear protein]]
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[[Category: phosphorylation]]
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[[Category: transcriptional corepressor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:15:44 2007''
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Current revision

Crystal structure of CtBP dehydrogenase core holo form

PDB ID 1mx3

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