1n0j

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(New page: 200px<br /> <applet load="1n0j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n0j, resolution 2.2&Aring;" /> '''The Structure of Hum...)
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[[Image:1n0j.gif|left|200px]]<br />
 
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<applet load="1n0j" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n0j, resolution 2.2&Aring;" />
 
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'''The Structure of Human Mitochondrial MN3+ Superoxide Dismutase Reveals a Novel Tetrameric Interface of Two 4-Helix Bundles'''<br />
 
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==Overview==
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==The Structure of Human Mitochondrial MN3+ Superoxide Dismutase Reveals a Novel Tetrameric Interface of Two 4-Helix Bundles==
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The 2.2 A resolution crystal structure of recombinant human manganese, superoxide dismutase, a homotetrameric enzyme that protects mitochondria, against oxygen-mediated free radical damage, has been determined. Within, each subunit, both the N-terminal helical hairpin and C-terminal, alpha/beta domains contribute ligands to the catalytic manganese site. Two, identical 4-helix bundles, symmetrically assembled from the N-terminal, helical hairpins, form novel tetrameric interfaces that stabilize the, active sites. Structurally altered polymorphic variants with reduced, activity, such as tetrameric interface mutant Ile-58 to Thr, may produce, not only an early selective advantage, through enhanced cytotoxicity of, tumor necrosis factor for virus-infected cells, but also detrimental, effects from increased mitochondrial oxidative damage, contributing to, degenerative conditions, including diabetes, aging, and Parkinson's and, Alzheimer's diseases.
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<StructureSection load='1n0j' size='340' side='right'caption='[[1n0j]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1n0j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1abm 1abm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N0J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N0J FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n0j OCA], [https://pdbe.org/1n0j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n0j RCSB], [https://www.ebi.ac.uk/pdbsum/1n0j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n0j ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/SODM_HUMAN SODM_HUMAN] Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:[https://omim.org/entry/612634 612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
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== Function ==
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[https://www.uniprot.org/uniprot/SODM_HUMAN SODM_HUMAN] Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.<ref>PMID:10334867</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n0/1n0j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n0j ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1N0J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. This structure superseeds the now removed PDB entry 1ABM. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1N0J OCA].
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*[[Superoxide dismutase 3D structures|Superoxide dismutase 3D structures]]
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== References ==
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==Reference==
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<references/>
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The structure of human mitochondrial manganese superoxide dismutase reveals a novel tetrameric interface of two 4-helix bundles., Borgstahl GE, Parge HE, Hickey MJ, Beyer WF Jr, Hallewell RA, Tainer JA, Cell. 1992 Oct 2;71(1):107-18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1394426 1394426]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Superoxide dismutase]]
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[[Category: Borgstahl GEO]]
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[[Category: Borgstahl, G.E.O.]]
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[[Category: Parge HE]]
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[[Category: Parge, H.E.]]
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[[Category: Tainer JA]]
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[[Category: Tainer, J.A.]]
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[[Category: MN]]
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[[Category: four-helix bundle]]
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[[Category: manganese]]
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[[Category: metalloenzyme]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:16:24 2007''
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Current revision

The Structure of Human Mitochondrial MN3+ Superoxide Dismutase Reveals a Novel Tetrameric Interface of Two 4-Helix Bundles

PDB ID 1n0j

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