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1mru

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[[Image:1mru.gif|left|200px]]
 
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==Intracellular Ser/Thr protein kinase domain of Mycobacterium tuberculosis PknB.==
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The line below this paragraph, containing "STRUCTURE_1mru", creates the "Structure Box" on the page.
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<StructureSection load='1mru' size='340' side='right'caption='[[1mru]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1mru]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MRU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MRU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_1mru| PDB=1mru | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mru FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mru OCA], [https://pdbe.org/1mru PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mru RCSB], [https://www.ebi.ac.uk/pdbsum/1mru PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mru ProSAT], [https://www.topsan.org/Proteins/TBSGC/1mru TOPSAN]</span></td></tr>
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</table>
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'''Intracellular Ser/Thr protein kinase domain of Mycobacterium tuberculosis PknB.'''
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== Function ==
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[https://www.uniprot.org/uniprot/PKNB_MYCTU PKNB_MYCTU] Key component of a signal transduction pathway that regulates cell growth and cell division via phosphorylation of target proteins such as GarA, GlmU, PapA5, PbpA, FhaB (Rv0019c), FhaA (Rv0020c), MviN, PstP, EmbR, Rv1422, Rv1747 and RseA. Shows a strong preference for Thr versus Ser as the phosphoacceptor.<ref>PMID:15985609</ref> <ref>PMID:15978616</ref> <ref>PMID:15987910</ref> <ref>PMID:16817899</ref> <ref>PMID:16980473</ref> <ref>PMID:16436437</ref> <ref>PMID:19826007</ref> <ref>PMID:19121323</ref> <ref>PMID:20025669</ref> <ref>PMID:21423706</ref> <ref>PMID:22275220</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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A family of eukaryotic-like Ser/Thr protein kinases occurs in bacteria, but little is known about the structures and functions of these proteins. Here we characterize PknB, a transmembrane signaling kinase from Mycobacterium tuberculosis. The intracellular PknB kinase domain is active autonomously, and the active enzyme is phosphorylated on residues homologous to regulatory phospho-acceptors in eukaryotic Ser/Thr kinases. The crystal structure of the PknB kinase domain in complex with an ATP analog reveals the active conformation. The predicted fold of the PknB extracellular domain matches the proposed targeting domain of penicillin-binding protein 2x. The structural and chemical similarities of PknB to metazoan homologs support a universal activation mechanism of Ser/Thr protein kinases in prokaryotes and eukaryotes.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mr/1mru_consurf.spt"</scriptWhenChecked>
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1MRU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MRU OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of Mycobacterium tuberculosis PknB supports a universal activation mechanism for Ser/Thr protein kinases., Young TA, Delagoutte B, Endrizzi JA, Falick AM, Alber T, Nat Struct Biol. 2003 Mar;10(3):168-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12548283 12548283]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mru ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Alber T]]
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[[Category: Alber, T.]]
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[[Category: Delagoutte B]]
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[[Category: Delagoutte, B.]]
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[[Category: Endrizzi JA]]
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[[Category: Endrizzi, J A.]]
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[[Category: Young TA]]
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[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: Young, T A.]]
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[[Category: Atp-recognition]]
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[[Category: Molecular evolution]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Regulatory]]
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[[Category: Structural genomic]]
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[[Category: Tb structural genomics consortium]]
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[[Category: Tbsgc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:38:40 2008''
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Current revision

Intracellular Ser/Thr protein kinase domain of Mycobacterium tuberculosis PknB.

PDB ID 1mru

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