1n3h
From Proteopedia
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(New page: 200px<br /> <applet load="1n3h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1n3h" /> '''Coupling of Folding and Binding in the PTB ...) |
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| - | [[Image:1n3h.gif|left|200px]]<br /> | ||
| - | <applet load="1n3h" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1n3h" /> | ||
| - | '''Coupling of Folding and Binding in the PTB Domain of the Signaling Protein Shc'''<br /> | ||
| - | == | + | ==Coupling of Folding and Binding in the PTB Domain of the Signaling Protein Shc== |
| - | The notion that certain proteins lack intrinsic globular structure under | + | <StructureSection load='1n3h' size='340' side='right'caption='[[1n3h]]' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1n3h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N3H FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3h OCA], [https://pdbe.org/1n3h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n3h RCSB], [https://www.ebi.ac.uk/pdbsum/1n3h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3h ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SHC1_HUMAN SHC1_HUMAN] Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.<ref>PMID:14665640</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n3/1n3h_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n3h ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The notion that certain proteins lack intrinsic globular structure under physiological conditions and that the attainment of fully folded structure only occurs upon the binding of target molecules has been recently gaining popularity. We report here the solution structure of the PTB domain of the signaling protein Shc in the free form. Comparison of this structure with that of the complex form, obtained previously with a phosphopeptide ligand, reveals that the Shc PTB domain is structurally disordered in the free form, particularly around the regions constituting the peptide binding pocket. The binding of the ligand appears to reorganize this pocket through local folding events triggering a conformational switch between the free and the complex forms. | ||
| - | + | Coupling of folding and binding in the PTB domain of the signaling protein Shc.,Farooq A, Zeng L, Yan KS, Ravichandran KS, Zhou MM Structure. 2003 Aug;11(8):905-13. PMID:12906822<ref>PMID:12906822</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 1n3h" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Farooq | + | [[Category: Farooq A]] |
| - | [[Category: Ravichandran | + | [[Category: Ravichandran KS]] |
| - | [[Category: Yan | + | [[Category: Yan KS]] |
| - | [[Category: Zeng | + | [[Category: Zeng L]] |
| - | [[Category: Zhou | + | [[Category: Zhou M-M]] |
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Current revision
Coupling of Folding and Binding in the PTB Domain of the Signaling Protein Shc
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