8wbp

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "8wbp" [edit=sysop:move=sysop])
Current revision (12:40, 23 October 2024) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
-
'''Unreleased structure'''
 
-
The entry 8wbp is ON HOLD
+
==Crystal structure of cis-Epoxysuccinate Hydrolases RhCESH[L] mutant E212Q==
 +
<StructureSection load='8wbp' size='340' side='right'caption='[[8wbp]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[8wbp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_opacus Rhodococcus opacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WBP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WBP FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wbp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wbp OCA], [https://pdbe.org/8wbp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wbp RCSB], [https://www.ebi.ac.uk/pdbsum/8wbp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wbp ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q1KLR5_RHOOP Q1KLR5_RHOOP]
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Microbial epoxide hydrolases, cis-epoxysuccinate hydrolases (CESHs), have been utilized for commercial production of enantiomerically pure L(+)- and D(-)-tartaric acids for decades. However, the stereo-catalytic mechanism of CESH producing L(+)-tartaric acid (CESH[L]) remains unclear. Herein, the crystal structures of two CESH[L]s in ligand-free, product-complexed, and catalytic intermediate forms were determined. These structures revealed the unique specific binding mode for the mirror-symmetric substrate, an active catalytic triad consisting of Asp-His-Glu, and an arginine providing a proton to the oxirane oxygen to facilitate the epoxide ring-opening reaction, which has been pursued for decades. These results provide the structural basis for the rational engineering of these industrial biocatalysts.
-
Authors: Dong, S., Xuan, J.S., Feng, Y.G., Cui, Q.
+
Deciphering the stereo-specific catalytic mechanisms of cis-epoxysuccinate hydrolases producing L(+)-tartaric acid.,Dong S, Xuan J, Feng Y, Cui Q J Biol Chem. 2024 Feb;300(2):105635. doi: 10.1016/j.jbc.2024.105635. Epub 2024 , Jan 8. PMID:38199576<ref>PMID:38199576</ref>
-
Description: Crystal structure of cis-Epoxysuccinate Hydrolases RhCESH[L] mutant E212Q
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Unreleased Structures]]
+
</div>
-
[[Category: Dong, S]]
+
<div class="pdbe-citations 8wbp" style="background-color:#fffaf0;"></div>
-
[[Category: Xuan, J.S]]
+
== References ==
-
[[Category: Cui, Q]]
+
<references/>
-
[[Category: Feng, Y.G]]
+
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Rhodococcus opacus]]
 +
[[Category: Cui Q]]
 +
[[Category: Dong S]]
 +
[[Category: Feng YG]]
 +
[[Category: Xuan JS]]

Current revision

Crystal structure of cis-Epoxysuccinate Hydrolases RhCESH[L] mutant E212Q

PDB ID 8wbp

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/8wbp"
Personal tools