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1mvf

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[[Image:1mvf.gif|left|200px]]
 
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==MazE addiction antidote==
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The line below this paragraph, containing "STRUCTURE_1mvf", creates the "Structure Box" on the page.
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<StructureSection load='1mvf' size='340' side='right'caption='[[1mvf]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1mvf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MVF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvf OCA], [https://pdbe.org/1mvf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mvf RCSB], [https://www.ebi.ac.uk/pdbsum/1mvf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvf ProSAT]</span></td></tr>
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{{STRUCTURE_1mvf| PDB=1mvf | SCENE= }}
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</table>
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== Function ==
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'''MazE addiction antidote'''
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[https://www.uniprot.org/uniprot/MAZE_ECOLI MAZE_ECOLI] Antitoxin component of a toxin-antitoxin (TA) module. Labile antitoxin that binds to the MazF mRNA interferase toxin and neutralizes its endoribonuclease activity. Is considered to be an 'addiction' molecule as the cell will die in its absence. The endoribonuclease activity (MazF, a toxin) is inhibited by the labile cognate antitoxin MazE. Toxicity results when the levels of MazE decrease in the cell, leading to mRNA degradation. This effect can be rescued by expression of MazE, but after 6 hours in rich medium the overexpression of MazF leads to programmed cell death. Cell growth and viability are not affected when MazF and MazE are coexpressed. Both MazE and MazE-MazF bind to the promoter region of the mazE-mazF operon to inhibit their transcription. Cell death governed by the MazE-MazF and DinJ-YafQ TA modules seems to play a role in biofilm formation, while MazE-MazF is also implicated in cell death in liquid media.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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A specific camel VHH (variable domain of dromedary heavy chain antibody) fragment was used to crystallize the intrinsically flexible addiction antidote MazE. Only 45% of the polypeptide chain is found ordered in the crystal. The MazE monomer consisting of two beta-hairpins connected by a short alpha-helix has no hydrophobic core on its own and represents only one half of a typical protein domain. A complete domain structure is formed by the association of two chains, creating a hydrophobic core between two four-stranded beta-sheets. This hydrophobic core consists exclusively of short aliphatic residues. The folded part of MazE contains a novel DNA binding motif. A model for DNA binding that is consistent with the available biochemical data is presented.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mv/1mvf_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1MVF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVF OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mvf ConSurf].
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Crystal structure of the intrinsically flexible addiction antidote MazE., Loris R, Marianovsky I, Lah J, Laeremans T, Engelberg-Kulka H, Glaser G, Muyldermans S, Wyns L, J Biol Chem. 2003 Jul 25;278(30):28252-7. Epub 2003 May 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12743116 12743116]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Camelus dromedarius]]
[[Category: Camelus dromedarius]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Engelberg-Kulka, H.]]
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[[Category: Engelberg-Kulka H]]
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[[Category: Glaser, G.]]
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[[Category: Glaser G]]
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[[Category: Laeremans, T.]]
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[[Category: Laeremans T]]
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[[Category: Lah, J.]]
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[[Category: Lah J]]
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[[Category: Loris, R.]]
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[[Category: Loris R]]
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[[Category: Marianovsky, I.]]
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[[Category: Marianovsky I]]
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[[Category: Muyldermans, S.]]
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[[Category: Muyldermans S]]
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[[Category: Wyns, L.]]
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[[Category: Wyns L]]
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[[Category: Addiction antidote]]
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[[Category: Camel antibody]]
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[[Category: Plasmid addiction]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:46:01 2008''
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Current revision

MazE addiction antidote

PDB ID 1mvf

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