1mvz

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[[Image:1mvz.gif|left|200px]]
 
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==NMR solution structure of a Bowman Birk inhibitor isolated from snail medic seeds (Medicago Scutellata)==
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The line below this paragraph, containing "STRUCTURE_1mvz", creates the "Structure Box" on the page.
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<StructureSection load='1mvz' size='340' side='right'caption='[[1mvz]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1mvz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_scutellata Medicago scutellata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MVZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvz OCA], [https://pdbe.org/1mvz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mvz RCSB], [https://www.ebi.ac.uk/pdbsum/1mvz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mvz ProSAT]</span></td></tr>
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{{STRUCTURE_1mvz| PDB=1mvz | SCENE= }}
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</table>
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== Function ==
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'''NMR solution structure of a Bowman Birk inhibitor isolated from snail medic seeds (Medicago Scutellata)'''
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[https://www.uniprot.org/uniprot/IBB_MEDSC IBB_MEDSC] Inhibits trypsin but not chymotrypsin. Inhibits the trypsin-like proteinase activity present in larvae of the crop pests Adoxophyes orana, Hyphantria cunea, Lobesia botrana and Ostrinia nubilalis.<ref>PMID:9014368</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The high-resolution three-dimensional structure of a Bowman Birk inhibitor, purified from snail medic seeds (Medicago scutellata) (MSTI), has been determined in solution by 1H NMR spectroscopy at pH 5.6 and 27 degrees C. The structure of MSTI comprises two distinct symmetric domains each composed of a three-stranded beta-sheet containing a VIb type loop, where the active sites are located. A characteristic geometry of three aromatic residues confers stability to this protein, and we observe that this feature is conserved in all the Bowman Birk inhibitors of known structure. The two active domains exhibit different conformational features: the second domain displays higher flexibility and hydrophobicity with respect to the first one, and these properties have been correlated to a lower trypsin inhibitory specificity, in agreement with titration studies that have shown a stoichiometric ratio MSTI:trypsin of 1:1.5. NMR analysis indicated that MSTI undergoes self-association at concentrations higher than 2 mM, and the residues involved in this mechanism are localized at opposite faces of the molecule, having the highest positive and negative potential, respectively, thus indicating that electrostatic intermolecular interactions are the driving forces for MSTI association. Most of the residues affected by self-association are highly conserved in BBIs from different seeds, suggesting a functional relevance for these charged superficial patches, possibly involved in the interaction with other enzymes or macromolecules, thus triggering anti-carcinogenic activity.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mv/1mvz_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1MVZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_scutellata Medicago scutellata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MVZ OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mvz ConSurf].
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Anticarcinogenic Bowman Birk inhibitor isolated from snail medic seeds (Medicago scutellata): solution structure and analysis of self-association behavior., Catalano M, Ragona L, Molinari H, Tava A, Zetta L, Biochemistry. 2003 Mar 18;42(10):2836-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12627949 12627949]
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Medicago scutellata]]
[[Category: Medicago scutellata]]
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[[Category: Single protein]]
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[[Category: Catalano M]]
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[[Category: Catalano, M.]]
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[[Category: Molinari H]]
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[[Category: Molinari, H.]]
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[[Category: Ragona L]]
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[[Category: Ragona, L.]]
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[[Category: Tava A]]
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[[Category: Tava, A.]]
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[[Category: Zetta L]]
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[[Category: Zetta, L.]]
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[[Category: Serine protease inhibitor]]
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[[Category: Three stranded beta-sheet]]
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[[Category: Vib type turn]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:47:12 2008''
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Current revision

NMR solution structure of a Bowman Birk inhibitor isolated from snail medic seeds (Medicago Scutellata)

PDB ID 1mvz

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