1mwk

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[[Image:1mwk.gif|left|200px]]
 
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==ParM from plasmid R1 APO form==
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The line below this paragraph, containing "STRUCTURE_1mwk", creates the "Structure Box" on the page.
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<StructureSection load='1mwk' size='340' side='right'caption='[[1mwk]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1mwk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwk OCA], [https://pdbe.org/1mwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mwk RCSB], [https://www.ebi.ac.uk/pdbsum/1mwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mwk ProSAT]</span></td></tr>
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{{STRUCTURE_1mwk| PDB=1mwk | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PARM_ECOLX PARM_ECOLX] Involved in the control of plasmid partition. Required for the accurate segregation of the plasmid.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mwk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mwk ConSurf].
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<div style="clear:both"></div>
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'''ParM from plasmid R1 APO form'''
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==See Also==
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*[[Plasmid segregation protein ParM|Plasmid segregation protein ParM]]
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__TOC__
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==Overview==
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</StructureSection>
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It was the general belief that DNA partitioning in prokaryotes is independent of a cytoskeletal structure, which in eukaryotic cells is indispensable for DNA segregation. Recently, however, immunofluorescence microscopy revealed highly dynamic, filamentous structures along the longitudinal axis of Escherichia coli formed by ParM, a plasmid-encoded protein required for accurate segregation of low-copy-number plasmid R1. We show here that ParM polymerizes into double helical protofilaments with a longitudinal repeat similar to filamentous actin (F-actin) and MreB filaments that maintain the cell shape of non-spherical bacteria. The crystal structure of ParM with and without ADP demonstrates that it is a member of the actin family of proteins and shows a domain movement of 25 degrees upon nucleotide binding. Furthermore, the crystal structure of ParM reveals major differences in the protofilament interface compared with F-actin, despite the similar arrangement of the subunits within the filaments. Thus, there is now evidence for cytoskeletal structures, formed by actin-like filaments that are involved in plasmid partitioning in E.coli.
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==About this Structure==
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1MWK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWK OCA].
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==Reference==
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F-actin-like filaments formed by plasmid segregation protein ParM., van den Ent F, Moller-Jensen J, Amos LA, Gerdes K, Lowe J, EMBO J. 2002 Dec 16;21(24):6935-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12486014 12486014]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Amos, L A.]]
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[[Category: Amos LA]]
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[[Category: Ent, F Van den.]]
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[[Category: Gerdes K]]
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[[Category: Gerdes, K.]]
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[[Category: Lowe J]]
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[[Category: Lowe, J.]]
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[[Category: Moller-Jensen J]]
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[[Category: Moller-Jensen, J.]]
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[[Category: Van den Ent F]]
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[[Category: Plasmid]]
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[[Category: Plasmid partition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:48:21 2008''
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Current revision

ParM from plasmid R1 APO form

PDB ID 1mwk

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