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1nb0

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(New page: 200px<br /> <applet load="1nb0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1nb0, resolution 1.70&Aring;" /> '''Crystal Structure o...)
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[[Image:1nb0.gif|left|200px]]<br />
 
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<applet load="1nb0" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nb0, resolution 1.70&Aring;" />
 
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'''Crystal Structure of Human Riboflavin Kinase'''<br />
 
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==Overview==
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==Crystal Structure of Human Riboflavin Kinase==
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Riboflavin kinase (RFK) is an essential enzyme catalyzing the, phosphorylation of riboflavin (vitamin B(2)) to form FMN, an obligatory, step in vitamin B(2) utilization and flavin cofactor synthesis. The, structure of human RFK revealed a six-stranded antiparallel beta barrel, core structurally similar to the riboflavin synthase/ferredoxin reductase, FAD binding domain fold. The binding site of an intrinsically bound MgADP, defines a novel nucleotide binding motif that encompasses a loop, a 3(10), helix, and a reverse turn followed by a short beta strand. This active, site loop forms an arch with ATP and riboflavin binding at the opposite, side and the phosphoryl transfer appears to occur through the hole, underneath the arch. The invariant residues Asn36 and Glu86 are implicated, in the catalysis.
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<StructureSection load='1nb0' size='340' side='right'caption='[[1nb0]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1nb0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NB0 FirstGlance]. <br>
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1NB0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Riboflavin_kinase Riboflavin kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.26 2.7.1.26] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NB0 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nb0 OCA], [https://pdbe.org/1nb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nb0 RCSB], [https://www.ebi.ac.uk/pdbsum/1nb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nb0 ProSAT]</span></td></tr>
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Crystal structure of human riboflavin kinase reveals a beta barrel fold and a novel active site arch., Karthikeyan S, Zhou Q, Mseeh F, Grishin NV, Osterman AL, Zhang H, Structure. 2003 Mar;11(3):265-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12623014 12623014]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIFK_HUMAN RIFK_HUMAN] Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nb/1nb0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nb0 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Riboflavin kinase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Grishin NV]]
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[[Category: Grishin, N.V.]]
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[[Category: Karthikeyan S]]
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[[Category: Karthikeyan, S.]]
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[[Category: Mseeh F]]
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[[Category: Mseeh, F.]]
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[[Category: Osterman AL]]
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[[Category: Osterman, A.L.]]
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[[Category: Zhang H]]
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[[Category: Zhang, H.]]
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[[Category: Zhou Q]]
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[[Category: Zhou, Q.]]
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[[Category: ADP]]
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[[Category: MG]]
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[[Category: beta barrel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:19:59 2007''
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Current revision

Crystal Structure of Human Riboflavin Kinase

PDB ID 1nb0

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