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1ncn

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(New page: 200px<br /> <applet load="1ncn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ncn, resolution 2.70&Aring;" /> '''the receptor-bindin...)
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[[Image:1ncn.gif|left|200px]]<br />
 
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<applet load="1ncn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ncn, resolution 2.70&Aring;" />
 
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'''the receptor-binding domain of human B7-2'''<br />
 
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==Overview==
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==the receptor-binding domain of human B7-2==
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B7-1 and B7-2 are homologous costimulatory ligands expressed on the, surfaces of antigen-presenting cells. Their interactions with CD28/CTLA-4, receptors expressed on T cell surfaces are crucial for the proper, regulation of T cell activity. B7-1 and B7-2 display distinct roles in, immune regulation, although they are usually considered to have redundant, functions. Here, we report the crystal structure of the receptor-binding, (Ig V-type) domain of human B7-2 at 2.7-A resolution. Structures of, unliganded and liganded B7-1 and B7-2 suggest a physical-chemical basis, for the observed functional similarities and differences between these two, costimulatory ligands. Of particular note, whereas the majority of the, residues mediating B7-1 dimerization are hydrophobic, the B7-2 dimer, observed in the B7-2/CTLA-4 complex displays a very hydrophilic dimer, interface. These differences provide a mechanism for preventing the, formation of B7-1/B7-2 heterodimers. The divergence at the putative dimer, interface is also consistent with the lower tendency of B7-2 to dimerize, as shown by the monomeric state of unliganded B7-2 both in solution and, crystalline form, and may result in detailed differences in signaling, mechanisms associated with B7-1 and B7-2.
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<StructureSection load='1ncn' size='340' side='right'caption='[[1ncn]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ncn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NCN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NCN FirstGlance]. <br>
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1NCN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NCN OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ncn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ncn OCA], [https://pdbe.org/1ncn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ncn RCSB], [https://www.ebi.ac.uk/pdbsum/1ncn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ncn ProSAT]</span></td></tr>
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==Reference==
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</table>
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Crystal structure of the receptor-binding domain of human B7-2: insights into organization and signaling., Zhang X, Schwartz JC, Almo SC, Nathenson SG, Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2586-91. Epub 2003 Feb 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12606712 12606712]
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== Function ==
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[https://www.uniprot.org/uniprot/CD86_HUMAN CD86_HUMAN] Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nc/1ncn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ncn ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Almo, S.C.]]
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[[Category: Almo SC]]
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[[Category: Nathenson, S.G.]]
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[[Category: Nathenson SG]]
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[[Category: Schwartz, J.D.]]
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[[Category: Schwartz JD]]
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[[Category: Zhang, X.]]
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[[Category: Zhang X]]
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[[Category: beta strands]]
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[[Category: ig v]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:20:25 2007''
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Current revision

the receptor-binding domain of human B7-2

PDB ID 1ncn

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