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1n0q

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[[Image:1n0q.gif|left|200px]]
 
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==3ANK: A designed ankyrin repeat protein with three identical consensus repeats==
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The line below this paragraph, containing "STRUCTURE_1n0q", creates the "Structure Box" on the page.
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<StructureSection load='1n0q' size='340' side='right'caption='[[1n0q]], [[Resolution|resolution]] 1.26&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1n0q]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N0Q FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.26&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TFA:TRIFLUOROACETIC+ACID'>TFA</scene></td></tr>
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{{STRUCTURE_1n0q| PDB=1n0q | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n0q OCA], [https://pdbe.org/1n0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n0q RCSB], [https://www.ebi.ac.uk/pdbsum/1n0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n0q ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n0/1n0q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n0q ConSurf].
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<div style="clear:both"></div>
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'''3ANK: A designed ankyrin repeat protein with three identical consensus repeats'''
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==See Also==
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*[[Ankyrin 3D structures|Ankyrin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The ankyrin repeat is one of the most common, modular, protein-protein interaction motifs in nature. To understand the structural determinants of this family of proteins and extract the consensus information that defines the architecture of this motif, we have designed a series of idealized ankyrin repeat proteins containing one, two, three, or four repeats by using statistical analysis of approximately 4,000 ankyrin repeat sequences from the PFAM database. Biophysical and x-ray crystallographic studies of the three and four repeat constructs (3ANK and 4ANK) to 1.26 and 1.5 A resolution, respectively, demonstrate that these proteins are well-folded, monomeric, display high thermostability, and adopt a very regular, tightly packed ankyrin repeat fold. Mapping the degree of amino acid conservation at each position on the 4ANK structure shows that most nonconserved residues are clustered on the surface of the molecule that has been designated as the binding site in naturally occurring ankyrin repeat proteins. Thus, the consensus amino acid sequence contains all information required to define the ankyrin repeat fold. Our results suggest that statistical analysis and the consensus sequence approach can be used as an effective method to design proteins with complex topologies. These generic ankyrin repeat proteins can serve as prototypes for dissecting the rules of molecular recognition mediated by ankyrin repeats and for engineering proteins with novel biological functions.
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[[Category: Large Structures]]
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[[Category: Minor Jr DL]]
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==About this Structure==
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[[Category: Mosavi LK]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N0Q OCA].
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[[Category: Peng Z-Y]]
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==Reference==
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Consensus-derived structural determinants of the ankyrin repeat motif., Mosavi LK, Minor DL Jr, Peng ZY, Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16029-34. Epub 2002 Dec 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12461176 12461176]
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[[Category: Jr., D L.Minor.]]
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[[Category: Mosavi, L K.]]
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[[Category: Peng, Z-Y.]]
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[[Category: Ank]]
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[[Category: Ankyrin repeat]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:56:33 2008''
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Current revision

3ANK: A designed ankyrin repeat protein with three identical consensus repeats

PDB ID 1n0q

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