8wbb
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==CryoEM structure of non-structural protein 1 dimer from dengue virus type 4== | |
| + | <StructureSection load='8wbb' size='340' side='right'caption='[[8wbb]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8wbb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_4_Philippines/H241/1956 Dengue virus 4 Philippines/H241/1956]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WBB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WBB FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wbb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wbb OCA], [https://pdbe.org/8wbb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wbb RCSB], [https://www.ebi.ac.uk/pdbsum/8wbb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wbb ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Flaviviruses encode a conserved, membrane-associated nonstructural protein 1 (NS1) with replication and immune evasion functions. The current knowledge of secreted NS1 (sNS1) oligomers is based on several low-resolution structures, thus hindering the development of drugs and vaccines against flaviviruses. Here, we revealed that recombinant sNS1 from flaviviruses exists in a dynamic equilibrium of dimer-tetramer-hexamer states. Two DENV4 hexameric NS1 structures and several tetrameric NS1 structures from multiple flaviviruses were solved at atomic resolution by cryo-EM. The stacking of the tetrameric NS1 and hexameric NS1 is facilitated by the hydrophobic beta-roll and connector domains. Additionally, a triacylglycerol molecule located within the central cavity may play a role in stabilizing the hexamer. Based on differentiated interactions between the dimeric NS1, two distinct hexamer models (head-to-head and side-to-side hexamer) and the step-by-step assembly mechanisms of NS1 dimer into hexamer were proposed. We believe that our study sheds light on the understanding of the NS1 oligomerization and contributes to NS1-based therapies. | ||
| - | + | The step-by-step assembly mechanism of secreted flavivirus NS1 tetramer and hexamer captured at atomic resolution.,Pan Q, Jiao H, Zhang W, Chen Q, Zhang G, Yu J, Zhao W, Hu H Sci Adv. 2024 May 3;10(18):eadm8275. doi: 10.1126/sciadv.adm8275. Epub 2024 May , 1. PMID:38691607<ref>PMID:38691607</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8wbb" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Dengue virus 4 Philippines/H241/1956]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Hu HL]] | ||
| + | [[Category: Jiao HZ]] | ||
| + | [[Category: Pan Q]] | ||
Current revision
CryoEM structure of non-structural protein 1 dimer from dengue virus type 4
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