1n1h

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:51, 14 February 2024) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1n1h.gif|left|200px]]
 
-
<!--
+
==Initiation complex of polymerase lambda3 from reovirus==
-
The line below this paragraph, containing "STRUCTURE_1n1h", creates the "Structure Box" on the page.
+
<StructureSection load='1n1h' size='340' side='right'caption='[[1n1h]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1n1h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3_Dearing Mammalian orthoreovirus 3 Dearing]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N1H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N1H FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CH1:3-DEOXY-CYTIDINE-5-TRIPHOSPHATE'>CH1</scene>, <scene name='pdbligand=G7M:N7-METHYL-GUANOSINE-5-MONOPHOSPHATE'>G7M</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GH3:3-DEOXY-GUANOSINE-5-TRIPHOSPHATE'>GH3</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-
{{STRUCTURE_1n1h| PDB=1n1h | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n1h OCA], [https://pdbe.org/1n1h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n1h RCSB], [https://www.ebi.ac.uk/pdbsum/1n1h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n1h ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RDRP_REOVD RDRP_REOVD] RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands.
-
'''Initiation complex of polymerase lambda3 from reovirus'''
+
==See Also==
-
 
+
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The reovirus polymerase and those of other dsRNA viruses function within the confines of a protein capsid to transcribe the tightly packed dsRNA genome segments. The crystal structure of the reovirus polymerase, lambda3, determined at 2.5 A resolution, shows a fingers-palm-thumb core, similar to those of other viral polymerases, surrounded by major N- and C-terminal elaborations, which create a cage-like structure, with four channels leading to the catalytic site. This "caged" polymerase has allowed us to visualize the results of several rounds of RNA polymerization directly in the crystals. A 5' cap binding site on the surface of lambda3 suggests a template retention mechanism by which attachment of the 5' end of the plus-sense strand facilitates insertion of the 3' end of the minus-sense strand into the template channel.
+
[[Category: Large Structures]]
-
 
+
[[Category: Mammalian orthoreovirus 3 Dearing]]
-
==About this Structure==
+
[[Category: Farsetta DL]]
-
1N1H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3 Mammalian orthoreovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N1H OCA].
+
[[Category: Harrison SC]]
-
 
+
[[Category: Nibert ML]]
-
==Reference==
+
[[Category: Tao Y]]
-
RNA synthesis in a cage--structural studies of reovirus polymerase lambda3., Tao Y, Farsetta DL, Nibert ML, Harrison SC, Cell. 2002 Nov 27;111(5):733-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12464184 12464184]
+
-
[[Category: Mammalian orthoreovirus 3]]
+
-
[[Category: Single protein]]
+
-
[[Category: Farsetta, D L.]]
+
-
[[Category: Harrison, S C.]]
+
-
[[Category: Nibert, M L.]]
+
-
[[Category: Tao, Y.]]
+
-
[[Category: Initiation complex]]
+
-
[[Category: Polymerase]]
+
-
[[Category: Right hand configuration]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:58:22 2008''
+

Current revision

Initiation complex of polymerase lambda3 from reovirus

PDB ID 1n1h

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n1h"
Personal tools