1n22

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[[Image:1n22.gif|left|200px]]
 
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==(+)-Bornyl Diphosphate Synthase: Complex with Mg, pyrophosphate, and (4R)-7-aza-7,8-dihydrolimonene==
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The line below this paragraph, containing "STRUCTURE_1n22", creates the "Structure Box" on the page.
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<StructureSection load='1n22' size='340' side='right'caption='[[1n22]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1n22]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salvia_officinalis Salvia officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N22 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N22 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7A8:(1R)-DIMETHYL-(4-METHYL-CYCLOHEX-3-ENYL)-AMINE'>7A8</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
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{{STRUCTURE_1n22| PDB=1n22 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n22 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n22 OCA], [https://pdbe.org/1n22 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n22 RCSB], [https://www.ebi.ac.uk/pdbsum/1n22 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n22 ProSAT]</span></td></tr>
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</table>
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'''(+)-Bornyl Diphosphate Synthase: Complex with Mg, pyrophosphate, and (4R)-7-aza-7,8-dihydrolimonene'''
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== Function ==
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[https://www.uniprot.org/uniprot/BPPS_SALOF BPPS_SALOF] Catalyzes the formation of the (+)-camphor precursor (+)-bornyl diphosphate from geranyl diphosphate. The enzyme also produces significant amounts of (+)-alpha-pinene, (+)-camphene, and (+-)-limonene.<ref>PMID:9614092</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The x-ray crystal structure of dimeric (+)-bornyl diphosphate synthase, a metal-requiring monoterpene cyclase from Salvia officinalis, is reported at 2.0-A resolution. Each monomer contains two alpha-helical domains: the C-terminal domain catalyzes the cyclization of geranyl diphosphate, orienting and stabilizing multiple reactive carbocation intermediates; the N-terminal domain has no clearly defined function, although its N terminus caps the active site in the C-terminal domain during catalysis. Structures of complexes with aza analogues of substrate and carbocation intermediates, as well as complexes with pyrophosphate and bornyl diphosphate, provide "snapshots" of the terpene cyclization cascade.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/1n22_consurf.spt"</scriptWhenChecked>
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1N22 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salvia_officinalis Salvia officinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N22 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Bornyl diphosphate synthase: structure and strategy for carbocation manipulation by a terpenoid cyclase., Whittington DA, Wise ML, Urbansky M, Coates RM, Croteau RB, Christianson DW, Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15375-80. Epub 2002 Nov 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12432096 12432096]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n22 ConSurf].
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[[Category: Geranyl-diphosphate cyclase]]
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Salvia officinalis]]
[[Category: Salvia officinalis]]
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[[Category: Single protein]]
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[[Category: Christianson DW]]
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[[Category: Christianson, D W.]]
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[[Category: Coates RM]]
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[[Category: Coates, R M.]]
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[[Category: Croteau RB]]
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[[Category: Croteau, R B.]]
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[[Category: Urbansky M]]
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[[Category: Urbansky, M.]]
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[[Category: Whittington DA]]
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[[Category: Whittington, D A.]]
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[[Category: Wise ML]]
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[[Category: Wise, M L.]]
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[[Category: Terpene synthase fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 01:59:31 2008''
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Current revision

(+)-Bornyl Diphosphate Synthase: Complex with Mg, pyrophosphate, and (4R)-7-aza-7,8-dihydrolimonene

PDB ID 1n22

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