1n2m

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[[Image:1n2m.jpg|left|200px]]
 
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==The S53A Proenzyme Structure of Methanococcus jannaschii.==
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The line below this paragraph, containing "STRUCTURE_1n2m", creates the "Structure Box" on the page.
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<StructureSection load='1n2m' size='340' side='right'caption='[[1n2m]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1n2m]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N2M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N2M FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
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{{STRUCTURE_1n2m| PDB=1n2m | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n2m OCA], [https://pdbe.org/1n2m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n2m RCSB], [https://www.ebi.ac.uk/pdbsum/1n2m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n2m ProSAT]</span></td></tr>
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</table>
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'''The S53A Proenzyme Structure of Methanococcus jannaschii.'''
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== Function ==
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[https://www.uniprot.org/uniprot/PDAD_METJA PDAD_METJA]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The three-dimensional structure of pyruvoyl-dependent arginine decarboxylase from Methanococcus jannaschii was determined at 1.4 A resolution. The pyruvoyl group of arginine decarboxylase is generated by an autocatalytic internal serinolysis reaction at Ser53 in the proenzyme resulting in two polypeptide chains. The structure of the nonprocessing S53A mutant was also determined. The active site of the processed enzyme unexpectedly contained the reaction product agmatine. The crystal structure confirms that arginine decarboxylase is a homotrimer. The protomer fold is a four-layer alphabetabetaalpha sandwich with topology similar to pyruvoyl-dependent histidine decarboxylase. Highly conserved residues Asn47, Ser52, Ser53, Ile54, and Glu109 are proposed to play roles in the self-processing reaction. Agmatine binding residues include the C terminus of the beta chain (Ser52) from one protomer and the Asp35 side chain and the Gly44 and Val46 carbonyl oxygen atoms from an adjacent protomer. Glu109 is proposed to play a catalytic role in the decarboxylation reaction.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/1n2m_consurf.spt"</scriptWhenChecked>
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1N2M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N2M OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Pyruvoyl-dependent arginine decarboxylase from Methanococcus jannaschii: crystal structures of the self-cleaved and S53A proenzyme forms., Tolbert WD, Graham DE, White RH, Ealick SE, Structure. 2003 Mar;11(3):285-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12623016 12623016]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n2m ConSurf].
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[[Category: Arginine decarboxylase]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
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[[Category: Single protein]]
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[[Category: Ealick SE]]
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[[Category: Ealick, S E.]]
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[[Category: Graham DE]]
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[[Category: Graham, D E.]]
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[[Category: Tolbert WD]]
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[[Category: Tolbert, W D.]]
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[[Category: White RH]]
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[[Category: White, R H.]]
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[[Category: Agmatine]]
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[[Category: Arginine decarboxylase]]
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[[Category: Pyruvate]]
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[[Category: Pyruvoyl group]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:00:50 2008''
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Current revision

The S53A Proenzyme Structure of Methanococcus jannaschii.

PDB ID 1n2m

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