1n35

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[[Image:1n35.gif|left|200px]]
 
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==lambda3 elongation complex with four phosphodiester bond formed==
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The line below this paragraph, containing "STRUCTURE_1n35", creates the "Structure Box" on the page.
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<StructureSection load='1n35' size='340' side='right'caption='[[1n35]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1n35]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3_Dearing Mammalian orthoreovirus 3 Dearing]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N35 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CH1:3-DEOXY-CYTIDINE-5-TRIPHOSPHATE'>CH1</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
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{{STRUCTURE_1n35| PDB=1n35 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n35 OCA], [https://pdbe.org/1n35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n35 RCSB], [https://www.ebi.ac.uk/pdbsum/1n35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n35 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RDRP_REOVD RDRP_REOVD] RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands.
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'''lambda3 elongation complex with four phosphodiester bond formed'''
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==See Also==
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The reovirus polymerase and those of other dsRNA viruses function within the confines of a protein capsid to transcribe the tightly packed dsRNA genome segments. The crystal structure of the reovirus polymerase, lambda3, determined at 2.5 A resolution, shows a fingers-palm-thumb core, similar to those of other viral polymerases, surrounded by major N- and C-terminal elaborations, which create a cage-like structure, with four channels leading to the catalytic site. This "caged" polymerase has allowed us to visualize the results of several rounds of RNA polymerization directly in the crystals. A 5' cap binding site on the surface of lambda3 suggests a template retention mechanism by which attachment of the 5' end of the plus-sense strand facilitates insertion of the 3' end of the minus-sense strand into the template channel.
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[[Category: Large Structures]]
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[[Category: Mammalian orthoreovirus 3 Dearing]]
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==About this Structure==
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[[Category: Farsetta DL]]
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1N35 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3 Mammalian orthoreovirus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N35 OCA].
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[[Category: Harrison SC]]
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[[Category: Nibert ML]]
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==Reference==
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[[Category: Tao Y]]
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RNA synthesis in a cage--structural studies of reovirus polymerase lambda3., Tao Y, Farsetta DL, Nibert ML, Harrison SC, Cell. 2002 Nov 27;111(5):733-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12464184 12464184]
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[[Category: Mammalian orthoreovirus 3]]
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[[Category: Single protein]]
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[[Category: Farsetta, D L.]]
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[[Category: Harrison, S C.]]
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[[Category: Nibert, M L.]]
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[[Category: Tao, Y.]]
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[[Category: Bracelet]]
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[[Category: Right hand configuration]]
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[[Category: Rna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:01:54 2008''
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Current revision

lambda3 elongation complex with four phosphodiester bond formed

PDB ID 1n35

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