This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1n8u

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:08, 3 April 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1n8u.jpg|left|200px]]
 
-
<!--
+
==Chemosensory Protein in Complex with bromo-dodecanol==
-
The line below this paragraph, containing "STRUCTURE_1n8u", creates the "Structure Box" on the page.
+
<StructureSection load='1n8u' size='340' side='right'caption='[[1n8u]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1n8u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mamestra_brassicae Mamestra brassicae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N8U FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDD:BROMO-DODECANOL'>BDD</scene></td></tr>
-
{{STRUCTURE_1n8u| PDB=1n8u | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n8u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n8u OCA], [https://pdbe.org/1n8u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n8u RCSB], [https://www.ebi.ac.uk/pdbsum/1n8u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n8u ProSAT]</span></td></tr>
-
 
+
</table>
-
'''Chemosensory Protein in Complex with bromo-dodecanol'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/Q9NG96_MAMBR Q9NG96_MAMBR]
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
Chemosensory proteins (CSPs) have been proposed to transport hydrophobic chemicals from air to olfactory or taste receptors. They have been isolated from several sensory organs of a wide range of insect species. The x-ray structure of CSPMbraA6, a 112-aa antennal protein from the moth Mamestra brassicae (Mbra), was shown to exhibit a novel type of alpha-helical fold. We have performed a structural and binding study of CSPMbraA6 to get some insights into its possible molecular function. Tryptophan fluorescence quenching demonstrates the ability of CSPMbraA6 to bind several types of semio-chemicals or surrogate ligands with microM K(d). Its crystal structure in complex with one of these compounds, 12-bromo-dodecanol, reveals extensive conformational changes on binding, resulting in the formation of a large cavity filled by three ligand molecules. Furthermore, binding cooperativity was demonstrated for some ligands, suggesting a stepwise binding. The peculiar rearrangement of CSPMbraA6 conformation and the cooperativity phenomenon might trigger the recognition of chemicals by receptors and induce subsequent signal transduction.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n8/1n8u_consurf.spt"</scriptWhenChecked>
-
1N8U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mamestra_brassicae Mamestra brassicae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8U OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
Moth chemosensory protein exhibits drastic conformational changes and cooperativity on ligand binding., Campanacci V, Lartigue A, Hallberg BM, Jones TA, Giudici-Orticoni MT, Tegoni M, Cambillau C, Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5069-74. Epub 2003 Apr 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12697900 12697900]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n8u ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Mamestra brassicae]]
[[Category: Mamestra brassicae]]
-
[[Category: Single protein]]
+
[[Category: Cambillau C]]
-
[[Category: Cambillau, C.]]
+
[[Category: Campanacci V]]
-
[[Category: Campanacci, V.]]
+
[[Category: Giudici-Orticoni MT]]
-
[[Category: Giudici-Orticoni, M T.]]
+
[[Category: Hallberg BM]]
-
[[Category: Hallberg, B M.]]
+
[[Category: Jones TA]]
-
[[Category: Jones, T A.]]
+
[[Category: Lartigue A]]
-
[[Category: Lartigue, A.]]
+
[[Category: Tegoni M]]
-
[[Category: Tegoni, M.]]
+
-
[[Category: Lipid binding protein]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:14:17 2008''
+

Current revision

Chemosensory Protein in Complex with bromo-dodecanol

PDB ID 1n8u

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n8u"
Personal tools