1n8w

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[[Image:1n8w.gif|left|200px]]
 
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==Biochemical and Structural Studies of Malate Synthase from Mycobacterium tuberculosis==
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The line below this paragraph, containing "STRUCTURE_1n8w", creates the "Structure Box" on the page.
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<StructureSection load='1n8w' size='340' side='right'caption='[[1n8w]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1n8w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N8W FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=GLV:GLYOXYLIC+ACID'>GLV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MLT:D-MALATE'>MLT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1n8w| PDB=1n8w | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n8w OCA], [https://pdbe.org/1n8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n8w RCSB], [https://www.ebi.ac.uk/pdbsum/1n8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n8w ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MASZ_MYCTU MASZ_MYCTU] Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA (By similarity).[HAMAP-Rule:MF_00641]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n8/1n8w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n8w ConSurf].
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<div style="clear:both"></div>
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'''Biochemical and Structural Studies of Malate Synthase from Mycobacterium tuberculosis'''
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==See Also==
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*[[Malate synthase 3D structures|Malate synthase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Establishment or maintenance of a persistent infection by Mycobacterium tuberculosis requires the glyoxylate pathway. This is a bypass of the tricarboxylic acid cycle in which isocitrate lyase and malate synthase (GlcB) catalyze the net incorporation of carbon during growth of microorganisms on acetate or fatty acids as the primary carbon source. The glcB gene from M. tuberculosis, which encodes malate synthase, was cloned, and GlcB was expressed in Escherichia coli. The influence of media conditions on expression in M. tuberculosis indicated that this enzyme is regulated differentially to isocitrate lyase. Purified GlcB had K(m) values of 57 and 30 microm for its substrates glyoxylate and acetyl coenzyme A, respectively, and was inhibited by bromopyruvate, oxalate, and phosphoenolpyruvate. The GlcB structure was solved to 2.1-A resolution in the presence of glyoxylate and magnesium. We also report the structure of GlcB in complex with the products of the reaction, coenzyme A and malate, solved to 2.7-A resolution. Coenzyme A binds in a bent conformation, and the details of its interactions are described, together with implications on the enzyme mechanism.
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[[Category: Large Structures]]
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==About this Structure==
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1N8W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8W OCA].
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==Reference==
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Biochemical and structural studies of malate synthase from Mycobacterium tuberculosis., Smith CV, Huang CC, Miczak A, Russell DG, Sacchettini JC, Honer zu Bentrup K, J Biol Chem. 2003 Jan 17;278(3):1735-43. Epub 2002 Oct 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12393860 12393860]
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[[Category: Malate synthase]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Bentrup, K Honer zu.]]
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[[Category: Huang, C C.]]
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[[Category: Miczak, A.]]
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[[Category: Russell, D G.]]
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[[Category: Sacchettini, J C.]]
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[[Category: Smith, C V.]]
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[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: Coenzyme some]]
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[[Category: Glcb]]
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[[Category: Glyoxylate]]
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[[Category: Glyoxylate pathway]]
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[[Category: Malate]]
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[[Category: Malate synthase]]
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[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Protein structure initiative]]
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[[Category: Honer zu Bentrup K]]
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[[Category: Psi]]
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[[Category: Huang CC]]
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[[Category: Structural genomic]]
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[[Category: Miczak A]]
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[[Category: Tb structural genomics consortium]]
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[[Category: Russell DG]]
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[[Category: Tbsgc]]
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[[Category: Sacchettini JC]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:14:24 2008''
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[[Category: Smith CV]]

Current revision

Biochemical and Structural Studies of Malate Synthase from Mycobacterium tuberculosis

PDB ID 1n8w

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