1n97

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[[Image:1n97.gif|left|200px]]
 
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==Crystal Structure of CYP175A1 from Thermus thermophillus strain HB27==
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The line below this paragraph, containing "STRUCTURE_1n97", creates the "Structure Box" on the page.
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<StructureSection load='1n97' size='340' side='right'caption='[[1n97]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1n97]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N97 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N97 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SRT:S,R+MESO-TARTARIC+ACID'>SRT</scene></td></tr>
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{{STRUCTURE_1n97| PDB=1n97 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n97 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n97 OCA], [https://pdbe.org/1n97 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n97 RCSB], [https://www.ebi.ac.uk/pdbsum/1n97 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n97 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q746J6_THET2 Q746J6_THET2]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n9/1n97_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n97 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The second structure of a thermophile cytochrome P450, CYP175A1 from the thermophilic bacterium Thermus thermophilus HB27, has been solved to 1.8-A resolution. The overall P450 structure remains conserved despite the low sequence identity between the various P450s. The CYP175A1 structure lacks the large aromatic network found in the only other thermostable P450, CYP119, thought to contribute to thermal stability. The primary difference between CYP175A1 and its mesophile counterparts is the investment of charged residues into salt-link networks at the expense of single charge-charge interactions. Additional factors involved in the thermal stability increase are a decrease in the overall size, especially shortening of loops and connecting regions, and a decrease in the number of labile residues such as Asn, Gln, and Cys.
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'''Crystal Stucture of CYP175A1 from Thermus thermophillus strain HB27'''
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Preliminary characterization and crystal structure of a thermostable cytochrome P450 from Thermus thermophilus.,Yano JK, Blasco F, Li H, Schmid RD, Henne A, Poulos TL J Biol Chem. 2003 Jan 3;278(1):608-16. Epub 2002 Oct 24. PMID:12401810<ref>PMID:12401810</ref>
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==Overview==
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The second structure of a thermophile cytochrome P450, CYP175A1 from the thermophilic bacterium Thermus thermophilus HB27, has been solved to 1.8-A resolution. The overall P450 structure remains conserved despite the low sequence identity between the various P450s. The CYP175A1 structure lacks the large aromatic network found in the only other thermostable P450, CYP119, thought to contribute to thermal stability. The primary difference between CYP175A1 and its mesophile counterparts is the investment of charged residues into salt-link networks at the expense of single charge-charge interactions. Additional factors involved in the thermal stability increase are a decrease in the overall size, especially shortening of loops and connecting regions, and a decrease in the number of labile residues such as Asn, Gln, and Cys.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1N97 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N97 OCA].
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</div>
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<div class="pdbe-citations 1n97" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Preliminary characterization and crystal structure of a thermostable cytochrome P450 from Thermus thermophilus., Yano JK, Blasco F, Li H, Schmid RD, Henne A, Poulos TL, J Biol Chem. 2003 Jan 3;278(1):608-16. Epub 2002 Oct 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12401810 12401810]
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*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
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[[Category: Single protein]]
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== References ==
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[[Category: Thermus thermophilus]]
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<references/>
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[[Category: Blasco, F.]]
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__TOC__
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[[Category: Henne, A.]]
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</StructureSection>
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[[Category: Li, H.]]
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[[Category: Large Structures]]
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[[Category: Poulos, T L.]]
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[[Category: Thermus thermophilus HB27]]
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[[Category: Schmid, R D.]]
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[[Category: Blasco F]]
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[[Category: Yano, J K.]]
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[[Category: Henne A]]
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[[Category: P450]]
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[[Category: Li H]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:15:10 2008''
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[[Category: Poulos TL]]
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[[Category: Schmid RD]]
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[[Category: Yano JK]]

Current revision

Crystal Structure of CYP175A1 from Thermus thermophillus strain HB27

PDB ID 1n97

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