3ajb
From Proteopedia
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== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/PEX3_HUMAN PEX3_HUMAN] Involved in peroxisome biosynthesis and integrity. Assembles membrane vesicles before the matrix proteins are translocated. As a docking factor for PEX19, is necessary for the import of peroxisomal membrane proteins in the peroxisomes.<ref>PMID:10848631</ref> <ref>PMID:15007061</ref> | [https://www.uniprot.org/uniprot/PEX3_HUMAN PEX3_HUMAN] Involved in peroxisome biosynthesis and integrity. Assembles membrane vesicles before the matrix proteins are translocated. As a docking factor for PEX19, is necessary for the import of peroxisomal membrane proteins in the peroxisomes.<ref>PMID:10848631</ref> <ref>PMID:15007061</ref> | ||
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| - | == Publication Abstract from PubMed == | ||
| - | Peroxisomes require peroxin (Pex) proteins for their biogenesis. The interaction between Pex3p, which resides on the peroxisomal membrane, and Pex19p, which resides in the cytosol, is crucial for peroxisome formation and the post-translational targeting of peroxisomal membrane proteins (PMPs). It is not known how Pex3p promotes the specific interaction with Pex19p for the purpose of PMP translocation. Here, we present the three-dimensional structure of the complex between a cytosolic domain of Pex3p and the binding-region peptide of Pex19p. The overall shape of Pex3p is a prolate spheroid with a novel fold, the 'twisted six-helix bundle.' The Pex19p-binding site is at an apex of the Pex3p spheroid. A 16-residue region of the Pex19p peptide forms an alpha-helix and makes a contact with Pex3p; this helix is disordered in the unbound state. The Pex19p peptide contains a characteristic motif, consisting of the leucine triad (Leu18, Leu21, Leu22), and Phe29, which are critical for the Pex3p binding and peroxisome biogenesis. | ||
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| - | Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p.,Sato Y, Shibata H, Nakatsu T, Nakano H, Kashiwayama Y, Imanaka T, Kato H EMBO J. 2010 Dec 15;29(24):4083-93. Epub 2010 Nov 19. PMID:21102411<ref>PMID:21102411</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
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| - | <div class="pdbe-citations 3ajb" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Current revision
Crystal Structure of human Pex3p in complex with N-terminal Pex19p peptide
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Categories: Homo sapiens | Large Structures | Kato H | Nakatsu T | Sato Y | Shibata H
