1nat

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:54, 14 February 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1nat.gif|left|200px]]
 
-
<!--
+
==CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS==
-
The line below this paragraph, containing "STRUCTURE_1nat", creates the "Structure Box" on the page.
+
<StructureSection load='1nat' size='340' side='right'caption='[[1nat]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1nat]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NAT FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nat OCA], [https://pdbe.org/1nat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nat RCSB], [https://www.ebi.ac.uk/pdbsum/1nat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nat ProSAT]</span></td></tr>
-
{{STRUCTURE_1nat| PDB=1nat | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SP0F_BACSU SP0F_BACSU] Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/na/1nat_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nat ConSurf].
 +
<div style="clear:both"></div>
-
'''CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS'''
+
==See Also==
-
 
+
*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]]
-
 
+
*[[Response regulator 3D structure|Response regulator 3D structure]]
-
==Overview==
+
__TOC__
-
Spo0F is a secondary messenger in the "two-component" system controlling the sporulation of Bacillus subtilis. Spo0F, like the chemotaxis protein CheY, is a single-domain protein homologous to the N-terminal activator domain of the response regulators. We recently reported the crystal structure of a phosphatase-resistant mutant Y13S of Spo0F with Ca2+ bound in the active site. The crystal structure of wild-type Spo0F in the absence of a metal ion is presented here. A comparison of the two structures reveals that the cation induces significant changes in the active site. In the present wild-type structure, the carboxylate of Asp11 points away from the center of the active site, whereas when coordinated to the Ca2+, as in the earlier structure, it points toward the active site. In addition, Asp54, the site of phosphorylation, is blocked by a salt bridge interaction of an Arg side chain from a neighboring molecule. From fluorescence quenching studies with Spo0F Y13W, we found that only the amino acid Arg binds to Spo0F in a saturable manner (Kd = 15 mM). This observation suggests that a small molecule with a shape complementary to the active site and having a guanidinium group might inhibit phosphotransfer between response regulators and their cognate histidine kinases.
+
</StructureSection>
-
 
+
-
==About this Structure==
+
-
1NAT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NAT OCA].
+
-
 
+
-
==Reference==
+
-
A response regulatory protein with the site of phosphorylation blocked by an arginine interaction: crystal structure of Spo0F from Bacillus subtilis., Madhusudan M, Zapf J, Hoch JA, Whiteley JM, Xuong NH, Varughese KI, Biochemistry. 1997 Oct 21;36(42):12739-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9335530 9335530]
+
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Hoch, J A.]]
+
[[Category: Hoch JA]]
[[Category: Madhusudan]]
[[Category: Madhusudan]]
-
[[Category: Varughese, K I.]]
+
[[Category: Varughese KI]]
-
[[Category: Whiteley, J M.]]
+
[[Category: Whiteley JM]]
-
[[Category: Xuong, N H.]]
+
[[Category: Xuong NH]]
-
[[Category: Zapf, J.]]
+
[[Category: Zapf J]]
-
[[Category: Aspartate pocket]]
+
-
[[Category: Regulatory protein]]
+
-
[[Category: Sporulation response regulator]]
+
-
[[Category: Two component system]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:18:20 2008''
+

Current revision

CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS

PDB ID 1nat

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nat"
Personal tools