8on7
From Proteopedia
(Difference between revisions)
| (One intermediate revision not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==FMRFa-bound Malacoceros FaNaC1 in lipid nanodiscs== | |
| + | <StructureSection load='8on7' size='340' side='right'caption='[[8on7]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8on7]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Malacoceros_fuliginosus Malacoceros fuliginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8ON7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8ON7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8on7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8on7 OCA], [https://pdbe.org/8on7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8on7 RCSB], [https://www.ebi.ac.uk/pdbsum/8on7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8on7 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Rapid signaling between neurons is mediated by ligand-gated ion channels, cell-surface proteins with an extracellular ligand-binding domain and a membrane-spanning ion channel domain. The degenerin/epithelial sodium channel (DEG/ENaC) superfamily is diverse in terms of its gating stimuli, with some DEG/ENaCs gated by neuropeptides, and others gated by pH, mechanical force or enzymatic activity. The mechanism by which ligands bind to and activate DEG/ENaCs is poorly understood. Here we dissected the structural basis for neuropeptide-gated activity of a neuropeptide-gated DEG/ENaC, FMRFamide-gated sodium channel 1 (FaNaC1) from the annelid worm Malacoceros fuliginosus, using cryo-electron microscopy. Structures of FaNaC1 in the ligand-free resting state and in several ligand-bound states reveal the ligand-binding site and capture the ligand-induced conformational changes of channel gating, which we verified with complementary mutagenesis experiments. Our results illuminate channel gating in DEG/ENaCs and offer a structural template for experimental dissection of channel pharmacology and ion conduction. | ||
| - | + | Structural basis for excitatory neuropeptide signaling.,Kalienkova V, Dandamudi M, Paulino C, Lynagh T Nat Struct Mol Biol. 2024 Apr;31(4):717-726. doi: 10.1038/s41594-023-01198-y. , Epub 2024 Feb 9. PMID:38337033<ref>PMID:38337033</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8on7" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Malacoceros fuliginosus]] | ||
| + | [[Category: Dandamudi M]] | ||
| + | [[Category: Kalienkova V]] | ||
| + | [[Category: Lynagh T]] | ||
| + | [[Category: Paulino C]] | ||
Current revision
FMRFa-bound Malacoceros FaNaC1 in lipid nanodiscs
| |||||||||||
