1ngw

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[[Image:1ngw.gif|left|200px]]
 
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==Chimeric Affinity Matured Fab 7g12 complexed with mesoporphyrin==
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The line below this paragraph, containing "STRUCTURE_1ngw", creates the "Structure Box" on the page.
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<StructureSection load='1ngw' size='340' side='right'caption='[[1ngw]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ngw]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NGW FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MMP:N-METHYLMESOPORPHYRIN'>MMP</scene></td></tr>
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{{STRUCTURE_1ngw| PDB=1ngw | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ngw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngw OCA], [https://pdbe.org/1ngw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ngw RCSB], [https://www.ebi.ac.uk/pdbsum/1ngw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ngw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HVM49_MOUSE HVM49_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ng/1ngw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ngw ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the Michaelis complex between the Fab fragment of ferrochelatase antibody 7G12 and its substrate mesoporphyrin has been solved to 2.6-A resolution. The antibody-bound mesoporphyrin clearly adopts a nonplanar conformation and reveals that the antibody catalyzes the porphyrin metallation reaction by straining/distorting the bound substrate toward the transition-state configuration. The crystal structures of the Fab fragment of the germ-line precursor antibody to 7G12 and its complex with the hapten N-methylmesoporphyrin have also been solved. A comparison of these structures with the corresponding structures of the affinity-matured antibody 7G12 reveals the molecular mechanism by which the immune system evolves binding energy to catalyze this reaction.
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'''Chimeric Affinity Matured Fab 7g12 complexed with mesoporphyrin'''
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Structural evidence for substrate strain in antibody catalysis.,Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:12552112<ref>PMID:12552112</ref>
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==Overview==
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The crystal structure of the Michaelis complex between the Fab fragment of ferrochelatase antibody 7G12 and its substrate mesoporphyrin has been solved to 2.6-A resolution. The antibody-bound mesoporphyrin clearly adopts a nonplanar conformation and reveals that the antibody catalyzes the porphyrin metallation reaction by straining/distorting the bound substrate toward the transition-state configuration. The crystal structures of the Fab fragment of the germ-line precursor antibody to 7G12 and its complex with the hapten N-methylmesoporphyrin have also been solved. A comparison of these structures with the corresponding structures of the affinity-matured antibody 7G12 reveals the molecular mechanism by which the immune system evolves binding energy to catalyze this reaction.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1NGW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus,_homo_sapiens Mus musculus, homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGW OCA].
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</div>
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<div class="pdbe-citations 1ngw" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12552112 12552112]
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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[[Category: Mus musculus, homo sapiens]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Andryski, S E.]]
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__TOC__
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[[Category: IV, A E.Beuscher.]]
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</StructureSection>
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[[Category: Schultz, P G.]]
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[[Category: Homo sapiens]]
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[[Category: Stevens, R C.]]
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[[Category: Large Structures]]
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[[Category: Yin, J.]]
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[[Category: Mus musculus]]
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[[Category: Antibody]]
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[[Category: Andryski SE]]
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[[Category: Antigen binding fragment]]
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[[Category: Beuscher IV AE]]
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[[Category: Immunoglobulin]]
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[[Category: Schultz PG]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:30:57 2008''
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[[Category: Stevens RC]]
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[[Category: Yin J]]

Current revision

Chimeric Affinity Matured Fab 7g12 complexed with mesoporphyrin

PDB ID 1ngw

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