1nj8

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[[Image:1nj8.gif|left|200px]]
 
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==Crystal Structure of Prolyl-tRNA Synthetase from Methanocaldococcus janaschii==
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The line below this paragraph, containing "STRUCTURE_1nj8", creates the "Structure Box" on the page.
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<StructureSection load='1nj8' size='340' side='right'caption='[[1nj8]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1nj8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NJ8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nj8 OCA], [https://pdbe.org/1nj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nj8 RCSB], [https://www.ebi.ac.uk/pdbsum/1nj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nj8 ProSAT]</span></td></tr>
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{{STRUCTURE_1nj8| PDB=1nj8 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SYP_METJA SYP_METJA] Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). Can inadvertently accommodate and process non-cognate amino acids such as cysteine and alanine.<ref>PMID:10642548</ref> <ref>PMID:10869184</ref> <ref>PMID:11141055</ref> <ref>PMID:11866507</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nj/1nj8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nj8 ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of Prolyl-tRNA Synthetase from Methanocaldococcus janaschii'''
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==See Also==
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*[[Aminoacyl tRNA synthetase 3D structures|Aminoacyl tRNA synthetase 3D structures]]
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== References ==
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==Overview==
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<references/>
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Cysteinyl-tRNA synthetase is an essential enzyme required for protein synthesis. Genes encoding this protein have not been identified in Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicus, or Methanopyrus kandleri. It has previously been proposed that the prolyl-tRNA synthetase (ProRS) enzymes in these organisms recognize either proline or cysteine and can aminoacylate their cognate tRNAs through a dual-specificity mechanism. We report five crystal structures at resolutions between 2.6 and 3.2 A: apo M. jannaschii ProRS, and M. thermautotrophicus ProRS in apo form and in complex with cysteinyl-sulfamoyl-, prolyl-sulfamoyl-, and alanyl-sulfamoyl-adenylates. These aminoacyl-adenylate analogues bind to a single active-site pocket and induce an identical set of conformational changes in loops around the active site when compared with the ligand-free conformation of ProRS. The cysteinyl- and prolyl-adenylate analogues have similar, nanomolar affinities for M. thermautotrophicus ProRS. Homology modeling of tRNA onto these adenylate complexes places the 3'-OH of A76 in an appropriate position for the transfer of any of the three amino acids to tRNA. Thus, these structures explain recent biochemical experiments showing that M. jannaschii ProRS misacylates tRNA(Pro) with cysteine, and argue against the proposal that these archaeal ProRS enzymes possess the dual capacity to aminoacylate both tRNA(Pro) and tRNA(Cys) with their cognate amino acids.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1NJ8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJ8 OCA].
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==Reference==
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The structural basis of cysteine aminoacylation of tRNAPro by prolyl-tRNA synthetases., Kamtekar S, Kennedy WD, Wang J, Stathopoulos C, Soll D, Steitz TA, Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1673-8. Epub 2003 Feb 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12578991 12578991]
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[[Category: Methanocaldococcus jannaschii]]
[[Category: Methanocaldococcus jannaschii]]
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[[Category: Proline--tRNA ligase]]
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[[Category: Kamtekar S]]
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[[Category: Single protein]]
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[[Category: Kennedy WD]]
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[[Category: Kamtekar, S.]]
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[[Category: Soll D]]
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[[Category: Kennedy, W D.]]
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[[Category: Stathopoulos C]]
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[[Category: Soll, D.]]
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[[Category: Steitz TA]]
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[[Category: Stathopoulos, C.]]
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[[Category: Wang J]]
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[[Category: Steitz, T A.]]
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[[Category: Wang, J.]]
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[[Category: Class-ii trna synthetase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:35:51 2008''
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Current revision

Crystal Structure of Prolyl-tRNA Synthetase from Methanocaldococcus janaschii

PDB ID 1nj8

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