1nkn

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[[Image:1nkn.gif|left|200px]]
 
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==VISUALIZING AN UNSTABLE COILED COIL: THE CRYSTAL STRUCTURE OF AN N-TERMINAL SEGMENT OF THE SCALLOP MYOSIN ROD==
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The line below this paragraph, containing "STRUCTURE_1nkn", creates the "Structure Box" on the page.
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<StructureSection load='1nkn' size='340' side='right'caption='[[1nkn]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1nkn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Argopecten_irradians Argopecten irradians] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NKN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NKN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nkn OCA], [https://pdbe.org/1nkn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nkn RCSB], [https://www.ebi.ac.uk/pdbsum/1nkn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nkn ProSAT]</span></td></tr>
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{{STRUCTURE_1nkn| PDB=1nkn | SCENE= }}
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</table>
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== Function ==
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'''VISUALIZING AN UNSTABLE COILED COIL: THE CRYSTAL STRUCTURE OF AN N-TERMINAL SEGMENT OF THE SCALLOP MYOSIN ROD'''
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[https://www.uniprot.org/uniprot/Q17042_ARGIR Q17042_ARGIR] [https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Alpha-helical coiled coils in muscle exemplify simplicity and economy of protein design: small variations in sequence lead to remarkable diversity in cellular functions. Myosin II is the key protein in muscle contraction, and the molecule's two-chain alpha-helical coiled-coil rod region--towards the carboxy terminus of the heavy chain--has unusual structural and dynamic features. The amino-terminal subfragment-2 (S2) domains of the rods can swing out from the thick filament backbone at a hinge in the coiled coil, allowing the two myosin 'heads' and their motor domains to interact with actin and generate tension. Most of the S2 rod appears to be a flexible coiled coil, but studies suggest that the structure at the N-terminal region is unstable, and unwinding or bending of the alpha-helices near the head-rod junction seems necessary for many of myosin's functional properties. Here we show the physical basis of a particularly weak coiled-coil segment by determining the 2.5-A-resolution crystal structure of a leucine-zipper-stabilized fragment of the scallop striated-muscle myosin rod adjacent to the head-rod junction. The N-terminal 14 residues are poorly ordered; the rest of the S2 segment forms a flexible coiled coil with poorly packed core residues. The unusual absence of interhelical salt bridges here exposes apolar core atoms to solvent.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nk/1nkn_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NKN OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nkn ConSurf].
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Visualization of an unstable coiled coil from the scallop myosin rod., Li Y, Brown JH, Reshetnikova L, Blazsek A, Farkas L, Nyitray L, Cohen C, Nature. 2003 Jul 17;424(6946):341-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12867988 12867988]
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<div style="clear:both"></div>
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[[Category: Blazsek, A.]]
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__TOC__
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[[Category: Brown, J H.]]
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</StructureSection>
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[[Category: Cohen, C.]]
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[[Category: Argopecten irradians]]
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[[Category: Farkas, L.]]
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[[Category: Large Structures]]
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[[Category: Li, Y.]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Nyitray, L.]]
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[[Category: Blazsek A]]
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[[Category: Reshetnikova, L.]]
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[[Category: Brown JH]]
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[[Category: Alpha helix]]
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[[Category: Cohen C]]
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[[Category: Coiled coil]]
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[[Category: Farkas L]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:38:43 2008''
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[[Category: Li Y]]
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[[Category: Nyitray L]]
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[[Category: Reshetnikova L]]

Current revision

VISUALIZING AN UNSTABLE COILED COIL: THE CRYSTAL STRUCTURE OF AN N-TERMINAL SEGMENT OF THE SCALLOP MYOSIN ROD

PDB ID 1nkn

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