8qwa
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Adenylosuccinate Synthetase from H. pylori in complex with PLP and IMP== | |
| + | <StructureSection load='8qwa' size='340' side='right'caption='[[8qwa]], [[Resolution|resolution]] 1.85Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8qwa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QWA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QWA FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qwa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qwa OCA], [https://pdbe.org/8qwa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qwa RCSB], [https://www.ebi.ac.uk/pdbsum/8qwa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qwa ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PURA_HELPY PURA_HELPY] Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.[HAMAP-Rule:MF_00011] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The current therapies against gastric pathogen Helicobacter pylori are ineffective in over 20% of patients. Enzymes belonging to the purine salvage pathway are considered as novel drug targets in this pathogen. Therefore, the main aim of the current study was to determine the antibacterial activity of pyridoxal 5'-phosphate (PLP), an active form of vitamin B6, against reference and clinical strains of H. pylori. Using a broad set of microbiological, physicochemical (UV absorption, LC-MS, X-ray analysis) and in silico experiments, we were able to prove that PLP inhibits adenylosuccinate synthetase (AdSS) from H. pylori by the competition with GTP (IC(50)(eq) approximately 30 nM). This behaviour was attributed to formation of a Schiff base with a lysine residue (a covalent bond with Lys322 in the GTP binding site of AdSS) and was potentiated by the presence of vitamin C. This antibacterial activity of PLP gives hope for its future use against H. pylori. | ||
| - | + | Vitamin B6 inhibits activity of Helicobacter pylori adenylosuccinate synthetase and growth of reference and clinical, antibiotic-resistant H. pylori strains.,Wojtys MI, Maksymiuk W, Narczyk M, Bubic A, Asler IL, Krzyzek P, Gosciniak G, Jagusztyn-Krynicka EK, Bzowska A J Enzyme Inhib Med Chem. 2024 Dec;39(1):2372734. doi: , 10.1080/14756366.2024.2372734. Epub 2024 Aug 16. PMID:39149761<ref>PMID:39149761</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8qwa" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Helicobacter pylori 26695]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bzowska A]] | ||
| + | [[Category: Maksymiuk W]] | ||
| + | [[Category: Narczyk M]] | ||
Current revision
Adenylosuccinate Synthetase from H. pylori in complex with PLP and IMP
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