8wrx
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Anti-CRISPR type II-A 28== | |
| + | <StructureSection load='8wrx' size='340' side='right'caption='[[8wrx]], [[Resolution|resolution]] 1.45Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8wrx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_phage_Javan128 Streptococcus phage Javan128]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8WRX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8WRX FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8wrx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8wrx OCA], [https://pdbe.org/8wrx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8wrx RCSB], [https://www.ebi.ac.uk/pdbsum/8wrx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8wrx ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | CRISPR-Cas systems serve as adaptive immune systems in bacteria and archaea, protecting against phages and other mobile genetic elements. However, phages and archaeal viruses have developed countermeasures, employing anti-CRISPR (Acr) proteins to counteract CRISPR-Cas systems. Despite the revolutionary impact of CRISPR-Cas systems on genome editing, concerns persist regarding potential off-target effects. Therefore, understanding the structural and molecular intricacies of diverse Acrs is crucial for elucidating the fundamental mechanisms governing CRISPR-Cas regulation. In this study, we present the structure of AcrIIA28 from Streptococcus phage Javan 128 and analyze its structural and functional features to comprehend the mechanisms involved in its inhibition of Cas9. Our current study reveals that AcrIIA28 is a metalloprotein that contains Zn2+ and abolishes the cleavage activity of Cas9 only from Streptococcus pyrogen (SpyCas9) by directly interacting with the REC3 domain of SpyCas9. Furthermore, we demonstrate that the AcrIIA28 interaction prevents the target DNA from being loaded onto Cas9. These findings indicate the molecular mechanisms underlying AcrIIA28-mediated Cas9 inhibition and provide valuable insights into the ongoing evolutionary battle between bacteria and phages. | ||
| - | + | AcrIIA28 is a metalloprotein that specifically inhibits targeted-DNA loading to SpyCas9 by binding to the REC3 domain.,Kim GE, Park HH Nucleic Acids Res. 2024 Jun 24;52(11):6459-6471. doi: 10.1093/nar/gkae357. PMID:38726868<ref>PMID:38726868</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8wrx" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptococcus phage Javan128]] | ||
| + | [[Category: Kim GE]] | ||
| + | [[Category: Park HH]] | ||
Current revision
Anti-CRISPR type II-A 28
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