This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1op8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1op8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1op8, resolution 2.5&Aring;" /> '''Crystal Structure of...)
Current revision (05:49, 17 April 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1op8.gif|left|200px]]<br />
 
-
<applet load="1op8" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1op8, resolution 2.5&Aring;" />
 
-
'''Crystal Structure of Human Granzyme A'''<br />
 
-
==Overview==
+
==Crystal Structure of Human Granzyme A==
-
Granzyme A (GzmA) belongs to a family of trypsin-like serine proteases, localized in cytoplasmic granules of activated lymphocytes and natural, killer (NK) cells. In contrast to the related granzyme B (GzmB), GzmA, forms a stable disulfide-linked homodimer and triggers target-cell death, in a caspase-independent way. Limited proteolysis of a high-molecular-mass, complex containing SET (also named putative HLA-associated protein II or, PHAPII), PHAPI (pp32, leucine-rich acidic nuclear protein) and HMG2 by, GzmA liberates NM23-H1, a Mg2+-dependent DNase that causes single-stranded, breaks in nuclear DNA. By analyzing the dimeric GzmA structure at a, resolution of 2.5 A, we determined the substrate-binding constraints and, selective advantages of the two domains arranged as a unique functional, tandem. The active sites of the two subunits point in opposite directions, and the nearby noncatalytic surfaces can function as exosites, presenting, substrates to the active site region of the adjacent partner in a manner, analogous to staphylokinase or streptokinase, which present plasminogen to, the cofactor-plasmin and cofactor-plasminogen complexes.
+
<StructureSection load='1op8' size='340' side='right'caption='[[1op8]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1op8]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OP8 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1op8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1op8 OCA], [https://pdbe.org/1op8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1op8 RCSB], [https://www.ebi.ac.uk/pdbsum/1op8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1op8 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/GRAA_HUMAN GRAA_HUMAN] This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis.<ref>PMID:12524539</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/op/1op8_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1op8 ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1OP8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Granzyme_A Granzyme A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.78 3.4.21.78] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OP8 OCA].
+
*[[Granzyme|Granzyme]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
Crystal structure of the apoptosis-inducing human granzyme A dimer., Hink-Schauer C, Estebanez-Perpina E, Kurschus FC, Bode W, Jenne DE, Nat Struct Biol. 2003 Jul;10(7):535-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12819770 12819770]
+
__TOC__
-
[[Category: Granzyme A]]
+
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Bode, W.]]
+
[[Category: Bode W]]
-
[[Category: Estebanez-Perpina, E.]]
+
[[Category: Estebanez-Perpina E]]
-
[[Category: Hink-Schauer, C.]]
+
[[Category: Hink-Schauer C]]
-
[[Category: Jenne, D.]]
+
[[Category: Jenne D]]
-
[[Category: SO4]]
+
-
[[Category: apoptosis]]
+
-
[[Category: granzyme a]]
+
-
[[Category: serine proteinase]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:35:03 2007''
+

Current revision

Crystal Structure of Human Granzyme A

PDB ID 1op8

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1op8"
Personal tools