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1nyt

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[[Image:1nyt.jpg|left|200px]]
 
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==SHIKIMATE DEHYDROGENASE AroE COMPLEXED WITH NADP+==
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The line below this paragraph, containing "STRUCTURE_1nyt", creates the "Structure Box" on the page.
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<StructureSection load='1nyt' size='340' side='right'caption='[[1nyt]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1nyt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NYT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NYT FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTV:(2S,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL'>DTV</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1nyt| PDB=1nyt | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nyt OCA], [https://pdbe.org/1nyt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nyt RCSB], [https://www.ebi.ac.uk/pdbsum/1nyt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nyt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AROE_ECOLI AROE_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ny/1nyt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nyt ConSurf].
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<div style="clear:both"></div>
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'''SHIKIMATE DEHYDROGENASE AroE COMPLEXED WITH NADP+'''
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==See Also==
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*[[Shikimate dehydrogenase 3D structures|Shikimate dehydrogenase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Shikimate dehydrogenase catalyzes the fourth step of the shikimate pathway, the essential route for the biosynthesis of aromatic compounds in plants and microorganisms. Absent in metazoans, this pathway is an attractive target for nontoxic herbicides and drugs. Escherichia coli expresses two shikimate dehydrogenase paralogs, the NADP-specific AroE and a putative enzyme YdiB. Here we characterize YdiB as a dual specificity quinate/shikimate dehydrogenase that utilizes either NAD or NADP as a cofactor. Structures of AroE and YdiB with bound cofactors were determined at 1.5 and 2.5 A resolution, respectively. Both enzymes display a similar architecture with two alpha/beta domains separated by a wide cleft. Comparison of their dinucleotide-binding domains reveals the molecular basis for cofactor specificity. Independent molecules display conformational flexibility suggesting that a switch between open and closed conformations occurs upon substrate binding. Sequence analysis and structural comparison led us to propose the catalytic machinery and a model for 3-dehydroshikimate recognition. Furthermore, we discuss the evolutionary and metabolic implications of the presence of two shikimate dehydrogenases in E. coli and other organisms.
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==About this Structure==
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1NYT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NYT OCA].
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==Reference==
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Structures of shikimate dehydrogenase AroE and its Paralog YdiB. A common structural framework for different activities., Michel G, Roszak AW, Sauve V, Maclean J, Matte A, Coggins JR, Cygler M, Lapthorn AJ, J Biol Chem. 2003 May 23;278(21):19463-72. Epub 2003 Mar 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12637497 12637497]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Shikimate dehydrogenase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Lapthorn AJ]]
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[[Category: Lapthorn, A J.]]
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[[Category: Roszak AW]]
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[[Category: Roszak, A W.]]
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[[Category: Alpha/beta domain]]
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[[Category: Wide cleft separation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:09:09 2008''
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Current revision

SHIKIMATE DEHYDROGENASE AroE COMPLEXED WITH NADP+

PDB ID 1nyt

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