1o06

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[[Image:1o06.gif|left|200px]]
 
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==Crystal structure of the Vps27p Ubiquitin Interacting Motif (UIM)==
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The line below this paragraph, containing "STRUCTURE_1o06", creates the "Structure Box" on the page.
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<StructureSection load='1o06' size='340' side='right'caption='[[1o06]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1o06]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O06 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O06 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1o06| PDB=1o06 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o06 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o06 OCA], [https://pdbe.org/1o06 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o06 RCSB], [https://www.ebi.ac.uk/pdbsum/1o06 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o06 ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of the Vps27p Ubiquitin Interacting Motif (UIM)'''
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== Function ==
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[https://www.uniprot.org/uniprot/VPS27_YEAST VPS27_YEAST] Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane. Controls exit from the prevacuolar compartment (PVC) in both the forward direction to the vacuole and the return to the Golgi. Allows VPS10 to return to the (trans-Golgi network) TGN from the PVC. Might also function as an alternate adapter in the COPIb clathrin-like coat.<ref>PMID:3062374</ref> <ref>PMID:1493335</ref> <ref>PMID:8649377</ref> <ref>PMID:9015300</ref> <ref>PMID:9265642</ref> <ref>PMID:11208109</ref> <ref>PMID:11416128</ref> <ref>PMID:12055639</ref> <ref>PMID:11872141</ref> <ref>PMID:12900393</ref> <ref>PMID:14581452</ref> <ref>PMID:15166140</ref> <ref>PMID:15107463</ref> <ref>PMID:15086794</ref> <ref>PMID:17101773</ref> <ref>PMID:17135292</ref>
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== References ==
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==Overview==
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<references/>
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Ubiquitylation is used to target proteins into a large number of different biological processes including proteasomal degradation, endocytosis, virus budding, and vacuolar protein sorting (Vps). Ubiquitylated proteins are typically recognized using one of several different conserved ubiquitin binding modules. Here, we report the crystal structure and ubiquitin binding properties of one such module, the ubiquitin-interacting motif (UIM). We found that UIM peptides from several proteins involved in endocytosis and vacuolar protein sorting including Hrs, Vps27p, Stam1, and Eps15 bound specifically, but with modest affinity (Kd = 0.1-1 mm), to free ubiquitin. Full affinity ubiquitin binding required the presence of conserved acidic patches at the N and C terminus of the UIM, as well as highly conserved central alanine and serine residues. NMR chemical shift perturbation mapping experiments demonstrated that all of these UIM peptides bind to the I44 surface of ubiquitin. The 1.45 A resolution crystal structure of the second yeast Vps27p UIM (Vps27p-2) revealed that the ubiquitin-interacting motif forms an amphipathic helix. Although Vps27p-2 is monomeric in solution, the motif unexpectedly crystallized as an antiparallel four-helix bundle, and the potential biological implications of UIM oligomerization are therefore discussed.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1O06 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O06 OCA].
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Alam SL]]
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==Reference==
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[[Category: Fisher RD]]
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Structure and ubiquitin binding of the ubiquitin-interacting motif., Fisher RD, Wang B, Alam SL, Higginson DS, Robinson H, Sundquist WI, Hill CP, J Biol Chem. 2003 Aug 1;278(31):28976-84. Epub 2003 May 14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12750381 12750381]
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[[Category: Higginson DS]]
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[[Category: Single protein]]
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[[Category: Hill CP]]
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[[Category: Alam, S L.]]
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[[Category: Myszka D]]
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[[Category: Fisher, R D.]]
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[[Category: Rich R]]
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[[Category: Higginson, D S.]]
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[[Category: Sundquist WI]]
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[[Category: Hill, C P.]]
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[[Category: Wang B]]
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[[Category: Myszka, D.]]
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[[Category: Rich, R.]]
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[[Category: Sundquist, W I.]]
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[[Category: Wang, B.]]
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[[Category: Alpha-helix]]
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[[Category: Coiled-coil]]
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[[Category: Tetramer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:12:03 2008''
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Current revision

Crystal structure of the Vps27p Ubiquitin Interacting Motif (UIM)

PDB ID 1o06

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