1o89

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[[Image:1o89.gif|left|200px]]
 
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==Crystal structure of E. COLI K-12 yhdH==
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The line below this paragraph, containing "STRUCTURE_1o89", creates the "Structure Box" on the page.
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<StructureSection load='1o89' size='340' side='right'caption='[[1o89]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1o89]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O89 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O89 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o89 OCA], [https://pdbe.org/1o89 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o89 RCSB], [https://www.ebi.ac.uk/pdbsum/1o89 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o89 ProSAT]</span></td></tr>
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{{STRUCTURE_1o89| PDB=1o89 | SCENE= }}
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</table>
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== Function ==
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'''CRYSTAL STRUCTURE OF E. COLI K-12 YHDH'''
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[https://www.uniprot.org/uniprot/ACUI_ECOLI ACUI_ECOLI] Probably catalyzes the NADPH-dependent reduction of acrylyl-CoA to propanoyl-CoA.<ref>PMID:22563425</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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As part of a structural genomics project on bacterial gene products of unknown function, the crystal structures of YhdH, a putative quinone oxidoreductase, and its complex with NADP have been determined at 2.25 and 2.6 A resolution, respectively. The overall fold of YhdH is very similar to that of alcohol dehydrogenases and quinone reductases despite its low sequence identity. The absence of any Zn ion indicates that YdhH is a putative quinone oxidoreductase. YhdH forms a homodimer, with each subunit composed of two domains: a catalytic domain and a coenzyme-binding domain. NADP is bound in a deep cleft formed between the two domains. Large conformational changes occur upon NADP binding, with the two domains closing up to each other and narrowing the NADP-binding cleft. Comparisons of the YdhH active site with those of the quinone oxidoreductases from Escherichia coli and Thermus thermophilus made it possible to identify essential conserved residues as being Asn41, Asp43, Asp64 and Arg318. The active-site size is very narrow and unless an induced fit occurs is accessible only to reagents the size of benzoquinone.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o8/1o89_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1O89 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O89 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1o89 ConSurf].
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Structure of Escherichia coli YhdH, a putative quinone oxidoreductase., Sulzenbacher G, Roig-Zamboni V, Pagot F, Grisel S, Salomoni A, Valencia C, Campanacci V, Vincentelli R, Tegoni M, Eklund H, Cambillau C, Acta Crystallogr D Biol Crystallogr. 2004 Oct;60(Pt 10):1855-62. Epub 2004, Sep 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15388933 15388933]
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<div style="clear:both"></div>
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[[Category: Escherichia coli]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Bignon, C.]]
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__TOC__
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[[Category: Cambillau, C.]]
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</StructureSection>
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[[Category: Grisel, S.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Pagot, F.]]
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[[Category: Large Structures]]
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[[Category: Roig-Zamboni, V.]]
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[[Category: Bignon C]]
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[[Category: Salamoni, A.]]
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[[Category: Cambillau C]]
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[[Category: Sulzenbacher, G.]]
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[[Category: Grisel S]]
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[[Category: Tegoni, M.]]
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[[Category: Pagot F]]
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[[Category: Valencia, C.]]
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[[Category: Roig-Zamboni V]]
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[[Category: Vincentelli, R.]]
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[[Category: Salamoni A]]
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[[Category: Possible nadph-dependent quinone oxidoreductase]]
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[[Category: Sulzenbacher G]]
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[[Category: Structural genomic]]
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[[Category: Tegoni M]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:30:41 2008''
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[[Category: Valencia C]]
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[[Category: Vincentelli R]]

Current revision

Crystal structure of E. COLI K-12 yhdH

PDB ID 1o89

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