1p9o

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(New page: 200px<br /> <applet load="1p9o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1p9o, resolution 2.30&Aring;" /> '''Crystal Structure o...)
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[[Image:1p9o.gif|left|200px]]<br />
 
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<applet load="1p9o" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p9o, resolution 2.30&Aring;" />
 
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'''Crystal Structure of Phosphopantothenoylcysteine Synthetase'''<br />
 
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==Overview==
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==Crystal Structure of Phosphopantothenoylcysteine Synthetase==
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The structure of human phosphopantothenoylcysteine (PPC) synthetase was, determined at 2.3 A resolution. PPC synthetase is a dimer with identical, monomers. Some features of the monomer fold resemble a group of, NAD-dependent enzymes, while other features resemble the ribokinase fold., The ATP, phosphopantothenate, and cysteine binding sites were deduced from, modeling studies. Highly conserved ATP binding residues include Gly43, Ser61, Gly63, Gly66, Phe230, and Asn258. Highly conserved, phosphopantothenate binding residues include Asn59, Ala179, Ala180, and, Asp183 from one monomer and Arg55' from the adjacent monomer. The, structure predicts a ping pong mechanism with initial formation of an, acyladenylate intermediate, followed by release of pyrophosphate and, attack by cysteine to form the final products PPC and AMP.
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<StructureSection load='1p9o' size='340' side='right'caption='[[1p9o]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1p9o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P9O FirstGlance]. <br>
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1P9O is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phosphopantothenate--cysteine_ligase Phosphopantothenate--cysteine ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.5 6.3.2.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1P9O OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9o OCA], [https://pdbe.org/1p9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p9o RCSB], [https://www.ebi.ac.uk/pdbsum/1p9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p9o ProSAT]</span></td></tr>
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Structure of human phosphopantothenoylcysteine synthetase at 2.3 A resolution., Manoj N, Strauss E, Begley TP, Ealick SE, Structure. 2003 Aug;11(8):927-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12906824 12906824]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PPCS_HUMAN PPCS_HUMAN] Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine.<ref>PMID:11923312</ref> <ref>PMID:12906824</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/1p9o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p9o ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Phosphopantothenate--cysteine ligase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Begley TP]]
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[[Category: Begley, T.P.]]
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[[Category: Ealick SE]]
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[[Category: Ealick, S.E.]]
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[[Category: Manoj N]]
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[[Category: Manoj, N.]]
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[[Category: Strauss E]]
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[[Category: Strauss, E.]]
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[[Category: SO4]]
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[[Category: synthetase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:41:33 2007''
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Current revision

Crystal Structure of Phosphopantothenoylcysteine Synthetase

PDB ID 1p9o

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