7dig
From Proteopedia
(Difference between revisions)
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<StructureSection load='7dig' size='340' side='right'caption='[[7dig]], [[Resolution|resolution]] 2.60Å' scene=''> | <StructureSection load='7dig' size='340' side='right'caption='[[7dig]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'> | + | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DIG FirstGlance]. <br> |
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene></td></tr> | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dig OCA], [https://pdbe.org/7dig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dig RCSB], [https://www.ebi.ac.uk/pdbsum/7dig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dig ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dig OCA], [https://pdbe.org/7dig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dig RCSB], [https://www.ebi.ac.uk/pdbsum/7dig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dig ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | == Function == | ||
| - | [https://www.uniprot.org/uniprot/Q8T6U0_9CNID Q8T6U0_9CNID] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Sensitive and accurate detection of specific metal ions is important for sensor development and can advance analytical science and support environmental and human medical examinations. Fluorescent proteins (FPs) can be quenched by specific metal ions and spectroscopically show a unique fluorescence-quenching sensitivity, suggesting their potential application as FP-based metal biosensors. Since the characteristics of the fluorescence quenching are difficult to predict, spectroscopic analysis of new FPs is important for the development of FP-based biosensors. Here we reported the spectroscopic and structural analysis of metal-induced fluorescence quenching of the photoconvertible fluorescent protein DendFP. The spectroscopic analysis showed that Fe(2+), Fe(3+), and Cu(2+) significantly reduced the fluorescence emission of DendFP. The metal titration experiments showed that the dissociation constants (Kd) of Fe(2+), Fe(3+), and Cu(2+) for DendFP were 24.59, 41.66, and 137.18 muM, respectively. The tetrameric interface of DendFP, which the metal ions cannot bind to, was analyzed. Structural comparison of the metal-binding sites of DendFP with those of iq-mEmerald and Dronpa suggested that quenchable DendFP has a unique metal-binding site on the beta-barrel that does not utilize the histidine pair for metal binding. | ||
| - | |||
| - | Metal-Induced Fluorescence Quenching of Photoconvertible Fluorescent Protein DendFP.,Kim IJ, Xu Y, Nam KH Molecules. 2022 May 3;27(9). pii: molecules27092922. doi:, 10.3390/molecules27092922. PMID:35566273<ref>PMID:35566273</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 7dig" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] | *[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Dendronephthya sp. SSAL-2002]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Nam KH]] | [[Category: Nam KH]] | ||
Current revision
Green fluorescent protein from Dendronephthya sp. SSAL-2002
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