This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1of3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (05:46, 17 April 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1of3.jpg|left|200px]]
 
-
<!--
+
==Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate-binding module, TmCBM27==
-
The line below this paragraph, containing "STRUCTURE_1of3", creates the "Structure Box" on the page.
+
<StructureSection load='1of3' size='340' side='right'caption='[[1of3]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1of3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OF3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OF3 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-
{{STRUCTURE_1of3| PDB=1of3 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1of3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1of3 OCA], [https://pdbe.org/1of3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1of3 RCSB], [https://www.ebi.ac.uk/pdbsum/1of3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1of3 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q9RIK9_THEMT Q9RIK9_THEMT]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/1of3_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1of3 ConSurf].
 +
<div style="clear:both"></div>
-
'''STRUCTURAL AND THERMODYNAMIC DISSECTION OF SPECIFIC MANNAN RECOGNITION BY A CARBOHYDRATE-BINDING MODULE, TMCBM27'''
+
==See Also==
-
 
+
*[[Mannosidase 3D structures|Mannosidase 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The C-terminal 176 amino acids of a Thermotoga maritima mannanase (Man5) constitute a carbohydrate binding module (CBM) that has been classified into CBM family 27. The isolated CBM27 domain, named TmCBM27, binds tightly (K(a)s 10(5)-10(6) M(-1)) to beta-1, 4-mannooligosaccharides, carob galactomannan, and konjac glucomannan, but not to cellulose (insoluble and soluble) or soluble birchwood xylan. The X-ray crystal structures of native TmCBM27, a TmCBM27-mannohexaose complex, and a TmCBM27-6(3),6(4)-alpha-D-galactosyl-mannopentaose complex at 2.0 A, 1.6 A, and 1.35 A, respectively, reveal the basis of TmCBM27's specificity for mannans. In particular, the latter complex, which is the first structure of a CBM in complex with a branched plant cell wall polysaccharide, illustrates how the architecture of the binding site can influence the recognition of naturally substituted polysaccharides.
+
[[Category: Large Structures]]
-
 
+
[[Category: Thermotoga maritima MSB8]]
-
==About this Structure==
+
[[Category: Boraston AB]]
-
1OF3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OF3 OCA].
+
[[Category: Boraston CM]]
-
 
+
[[Category: Davies GJ]]
-
==Reference==
+
[[Category: Nurizzo D]]
-
Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate binding module, TmCBM27., Boraston AB, Revett TJ, Boraston CM, Nurizzo D, Davies GJ, Structure. 2003 Jun;11(6):665-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12791255 12791255]
+
[[Category: Revett TJ]]
-
[[Category: Single protein]]
+
-
[[Category: Thermotoga maritima]]
+
-
[[Category: Boraston, A B.]]
+
-
[[Category: Boraston, C M.]]
+
-
[[Category: Davies, G J.]]
+
-
[[Category: Nurizzo, D.]]
+
-
[[Category: Revett, T J.]]
+
-
[[Category: Carbohydrate binding module]]
+
-
[[Category: Hydrolase]]
+
-
[[Category: Mannan binding]]
+
-
[[Category: Polysaccharide degradation]]
+
-
[[Category: Tmcbm27]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:45:59 2008''
+

Current revision

Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate-binding module, TmCBM27

PDB ID 1of3

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1of3"
Personal tools