1ofu

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[[Image:1ofu.jpg|left|200px]]
 
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==Crystal structure of SulA:FtsZ from Pseudomonas aeruginosa==
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The line below this paragraph, containing "STRUCTURE_1ofu", creates the "Structure Box" on the page.
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<StructureSection load='1ofu' size='340' side='right'caption='[[1ofu]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ofu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OFU FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
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{{STRUCTURE_1ofu| PDB=1ofu | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ofu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ofu OCA], [https://pdbe.org/1ofu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ofu RCSB], [https://www.ebi.ac.uk/pdbsum/1ofu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ofu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FTSZ_PSEAE FTSZ_PSEAE] Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/1ofu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ofu ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF SULA:FTSZ FROM PSEUDOMONAS AERUGINOSA'''
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==See Also==
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*[[Cell division protein 3D structures|Cell division protein 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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SulA halts cell division in Escherichia coli by binding to the major component of the division machinery FtsZ. We have solved the crystal structure of SulA alone and in complex with FtsZ from Pseudomonas aeruginosa. SulA is expressed when the SOS response is induced. This is a mechanism to inhibit cell division and repair DNA in the event of DNA damage. FtsZ is a tubulin-like protein that forms polymers, with the active-site GTPase split across two monomers. One monomer provides the GTP-binding site and the other, through its T7 loop nucleotide hydrolysis. Our structures show that SulA is a dimer, and that SulA inhibits cell division neither by binding the nucleotide-binding site nor by inducing conformational changes in FtsZ. Instead, SulA binds the T7 loop surface of FtsZ, opposite the nucleotide-binding site, blocking polymer formation. These findings explain why GTP hydrolysis and polymer turnover are required for SulA inhibition.
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[[Category: Large Structures]]
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==About this Structure==
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1OFU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OFU OCA].
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==Reference==
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Crystal structure of the SOS cell division inhibitor SulA and in complex with FtsZ., Cordell SC, Robinson EJ, Lowe J, Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7889-94. Epub 2003 Jun 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12808143 12808143]
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[[Category: Protein complex]]
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[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Cordell, S C.]]
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[[Category: Cordell SC]]
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[[Category: Lowe, J.]]
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[[Category: Lowe J]]
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[[Category: Robinson, E J.H.]]
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[[Category: Robinson EJH]]
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[[Category: Bacterial cell division inhibitor]]
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[[Category: Ftsz]]
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[[Category: Sula protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:47:44 2008''
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Current revision

Crystal structure of SulA:FtsZ from Pseudomonas aeruginosa

PDB ID 1ofu

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