1ohg

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[[Image:1ohg.jpg|left|200px]]
 
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==STRUCTURE OF THE DSDNA BACTERIOPHAGE HK97 MATURE EMPTY CAPSID==
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The line below this paragraph, containing "STRUCTURE_1ohg", creates the "Structure Box" on the page.
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<SX load='1ohg' size='340' side='right' viewer='molstar' caption='[[1ohg]], [[Resolution|resolution]] 3.45&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ohg]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_HK97 Escherichia virus HK97]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1fh6 1fh6]. The September 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Citrate Synthase'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_9 10.2210/rcsb_pdb/mom_2007_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OHG FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1ohg| PDB=1ohg | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ohg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ohg OCA], [https://pdbe.org/1ohg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ohg RCSB], [https://www.ebi.ac.uk/pdbsum/1ohg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ohg ProSAT]</span></td></tr>
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</table>
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'''STRUCTURE OF THE DSDNA BACTERIOPHAGE HK97 MATURE EMPTY CAPSID'''
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== Function ==
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[https://www.uniprot.org/uniprot/CAPSD_BPHK7 CAPSD_BPHK7] Assembles to form an icosahedral capsid of 66 nm, with a T=7 laevo symmetry (PubMed:11000116, PubMed:21276801). Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function.<ref>PMID:11000116</ref> <ref>PMID:21276801</ref> <ref>PMID:7669350</ref> <ref>PMID:7723020</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The HK97 bacteriophage capsid is a unique example of macromolecular catenanes: interlocked rings of covalently attached protein subunits. The chain mail organization of the subunits stabilizes a particle in which the maximum thickness of the protein shell is 18A and the maximum diameter is 550A. The electron density has the appearance of a balloon illustrating the extraordinary strength conferred by the unique subunit organization. The refined structure shows novel qualities of the HK97 shell protein, gp5 that, together with the protease gp4, guides the assembly and maturation of the virion. Although gp5 forms hexamers and pentamers and the subunits exist in different structural environments, the tertiary structures of the seven protein molecules in the viral asymmetric unit are closely similar. The interactions of the subunits in the shell are exceptionally complex with each subunit interacting with nine other subunits. The interactions of the N-terminus released after gp5 cleavage appear important for organization of the loops that become crosslinked to the core of a neighboring subunit at the maturation. A comparison with a model of the Prohead II structure revealed that the surfaces of non-covalent contact between the monomers that build up hexamers/pentamers are completely redefined during maturation.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oh/1ohg_consurf.spt"</scriptWhenChecked>
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1OHG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk97 Enterobacteria phage hk97]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1fh6 1fh6]. The following page contains interesting information on the relation of 1OHG with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb93_1.html Citrate Synthase]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OHG OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The refined structure of a protein catenane: the HK97 bacteriophage capsid at 3.44 A resolution., Helgstrand C, Wikoff WR, Duda RL, Hendrix RW, Johnson JE, Liljas L, J Mol Biol. 2003 Dec 12;334(5):885-99. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14643655 14643655]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ohg ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</SX>
[[Category: Citrate Synthase]]
[[Category: Citrate Synthase]]
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[[Category: Enterobacteria phage hk97]]
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[[Category: Escherichia virus HK97]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Duda, R L.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Helgstrand, C.]]
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[[Category: Duda RL]]
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[[Category: Hendrix, R W.]]
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[[Category: Helgstrand C]]
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[[Category: Johnson, J E.]]
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[[Category: Hendrix RW]]
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[[Category: Liljas, L.]]
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[[Category: Johnson JE]]
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[[Category: Wikoff, W R.]]
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[[Category: Liljas L]]
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[[Category: Auto-catalytic cross-link]]
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[[Category: Wikoff WR]]
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[[Category: Bacteriophage]]
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[[Category: Capsid]]
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[[Category: Icosahedral virus]]
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[[Category: Virus]]
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[[Category: Virus/viral protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:51:07 2008''
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Current revision

STRUCTURE OF THE DSDNA BACTERIOPHAGE HK97 MATURE EMPTY CAPSID

1ohg, resolution 3.45Å

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