8qxq
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==PsiM in complex with SAH and psilocybin== | |
| + | <StructureSection load='8qxq' size='340' side='right'caption='[[8qxq]], [[Resolution|resolution]] 0.94Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8qxq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Psilocybe_cubensis Psilocybe cubensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8QXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8QXQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.94Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=X8Q:[3-[2-(dimethylamino)ethyl]-1~{H}-indol-4-yl]+dihydrogen+phosphate'>X8Q</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8qxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8qxq OCA], [https://pdbe.org/8qxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8qxq RCSB], [https://www.ebi.ac.uk/pdbsum/8qxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8qxq ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Psilocybin, the natural hallucinogen produced by Psilocybe ("magic") mushrooms, holds great promise for the treatment of depression and several other mental health conditions. The final step in the psilocybin biosynthetic pathway, dimethylation of the tryptophan-derived intermediate norbaeocystin, is catalysed by PsiM. Here we present atomic resolution (0.9 A) crystal structures of PsiM trapped at various stages of its reaction cycle, providing detailed insight into the SAM-dependent methylation mechanism. Structural and phylogenetic analyses suggest that PsiM derives from epitranscriptomic N(6)-methyladenosine writers of the METTL16 family, which is further supported by the observation that bound substrates physicochemically mimic RNA. Inherent limitations of the ancestral monomethyltransferase scaffold hamper the efficiency of psilocybin assembly and leave PsiM incapable of catalysing trimethylation to aeruginascin. The results of our study will support bioengineering efforts aiming to create novel variants of psilocybin with improved therapeutic properties. | ||
| - | + | Methyl transfer in psilocybin biosynthesis.,Hudspeth J, Rogge K, Dorner S, Mull M, Hoffmeister D, Rupp B, Werten S Nat Commun. 2024 Mar 28;15(1):2709. doi: 10.1038/s41467-024-46997-z. PMID:38548735<ref>PMID:38548735</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8qxq" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Psilocybe cubensis]] | ||
| + | [[Category: Hudspeth J]] | ||
| + | [[Category: Rupp B]] | ||
| + | [[Category: Werten S]] | ||
Current revision
PsiM in complex with SAH and psilocybin
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