1oks

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[[Image:1oks.jpg|left|200px]]
 
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==Crystal structure of the measles virus phosphoprotein XD domain==
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The line below this paragraph, containing "STRUCTURE_1oks", creates the "Structure Box" on the page.
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<StructureSection load='1oks' size='340' side='right'caption='[[1oks]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1oks]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Measles_morbillivirus Measles morbillivirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OKS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
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{{STRUCTURE_1oks| PDB=1oks | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oks OCA], [https://pdbe.org/1oks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oks RCSB], [https://www.ebi.ac.uk/pdbsum/1oks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oks ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHOSP_MEASE PHOSP_MEASE] Essential component of the RNA polymerase and the nascent chain assembly complex. Also required during RNA synthesis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ok/1oks_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oks ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF THE MEASLES VIRUS PHOSPHOPROTEIN XD DOMAIN'''
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==See Also==
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Measles virus is a negative-sense, single-stranded RNA virus belonging to the Mononegavirales order which comprises several human pathogens such as Ebola, Nipah, and Hendra viruses. The phosphoprotein of measles virus is a modular protein consisting of an intrinsically disordered N-terminal domain (Karlin, D., Longhi, S., Receveur, V., and Canard, B. (2002) Virology 296, 251-262) and of a C-terminal moiety (PCT) composed of alternating disordered and globular regions. We report the crystal structure of the extreme C-terminal domain (XD) of measles virus phosphoprotein (aa 459-507) at 1.8 A resolution. We have previously reported that the C-terminal domain of measles virus nucleoprotein, NTAIL, is intrinsically unstructured and undergoes induced folding in the presence of PCT (Longhi, S., Receveur-Brechot, V., Karlin, D., Johansson, K., Darbon, H., Bhella, D., Yeo, R., Finet, S., and Canard, B. (2003) J. Biol. Chem. 278, 18638-18648). Using far-UV circular dichroism, we show that within PCT, XD is the region responsible for the induced folding of NTAIL. The crystal structure of XD consists of three helices, arranged in an anti-parallel triple-helix bundle. The surface of XD formed between helices alpha2 and alpha3 displays a long hydrophobic cleft that might provide a complementary hydrophobic surface to embed and promote folding of the predicted alpha-helix of NTAIL. We present a tentative model of the interaction between XD and NTAIL. These results, beyond presenting the first measles virus protein structure, shed light both on the function of the phosphoprotein at the molecular level and on the process of induced folding.
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[[Category: Large Structures]]
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[[Category: Measles morbillivirus]]
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==About this Structure==
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[[Category: Bourhis J-M]]
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1OKS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Measles_virus Measles virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OKS OCA].
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[[Category: Cambillau C]]
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[[Category: Campanacci V]]
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==Reference==
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[[Category: Canard B]]
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Crystal structure of the measles virus phosphoprotein domain responsible for the induced folding of the C-terminal domain of the nucleoprotein., Johansson K, Bourhis JM, Campanacci V, Cambillau C, Canard B, Longhi S, J Biol Chem. 2003 Nov 7;278(45):44567-73. Epub 2003 Aug 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12944395 12944395]
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[[Category: Johansson K]]
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[[Category: Measles virus]]
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[[Category: Longhi S]]
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[[Category: RNA-directed RNA polymerase]]
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[[Category: Single protein]]
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[[Category: Bourhis, J M.]]
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[[Category: Cambillau, C.]]
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[[Category: Campanacci, V.]]
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[[Category: Canard, B.]]
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[[Category: Johansson, K.]]
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[[Category: Longhi, S.]]
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[[Category: Nucleocapsid]]
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[[Category: Phosphorylation.]]
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[[Category: Rna-directed rna polymerase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:58:41 2008''
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Current revision

Crystal structure of the measles virus phosphoprotein XD domain

PDB ID 1oks

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