1opx

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[[Image:1opx.jpg|left|200px]]
 
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==Crystal structure of the traffic ATPase (HP0525) of the Helicobacter pylori type IV secretion system bound by sulfate==
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The line below this paragraph, containing "STRUCTURE_1opx", creates the "Structure Box" on the page.
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<StructureSection load='1opx' size='340' side='right'caption='[[1opx]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1opx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OPX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OPX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1opx| PDB=1opx | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1opx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1opx OCA], [https://pdbe.org/1opx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1opx RCSB], [https://www.ebi.ac.uk/pdbsum/1opx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1opx ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of the traffic ATPase (HP0525) of the Helicobacter pylori type IV secretion system bound by sulfate'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q7BK04_HELPX Q7BK04_HELPX]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The coupling of ATP binding/hydrolysis to macromolecular secretion systems is crucial to the pathogenicity of Gram-negative bacteria. We reported previously the structure of the ADP-bound form of the hexameric traffic VirB11 ATPase of the Helicobacter pylori type IV secretion system (named HP0525), and proposed that it functions as a gating molecule at the inner membrane, cycling through closed and open forms regulated by ATP binding/hydrolysis. Here, we combine crystal structures with analytical ultracentrifugation experiments to show that VirB11 ATPases indeed function as dynamic hexameric assemblies. In the absence of nucleotide, the N-terminal domains exhibit a collection of rigid-body conformations. Nucleotide binding 'locks' the hexamer into a symmetric and compact structure. We propose that VirB11s use the mechanical leverage generated by such nucleotide-dependent conformational changes to facilitate the export of substrates or the assembly of the type IV secretion apparatus. Biochemical characterization of mutant forms of HP0525 coupled with electron microscopy and in vivo assays support such hypothesis, and establish the relevance of VirB11s ATPases as drug targets against pathogenic bacteria.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/op/1opx_consurf.spt"</scriptWhenChecked>
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1OPX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OPX OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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VirB11 ATPases are dynamic hexameric assemblies: new insights into bacterial type IV secretion., Savvides SN, Yeo HJ, Beck MR, Blaesing F, Lurz R, Lanka E, Buhrdorf R, Fischer W, Haas R, Waksman G, EMBO J. 2003 May 1;22(9):1969-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12727865 12727865]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1opx ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Helicobacter pylori 26695]]
[[Category: Helicobacter pylori 26695]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Beck, M R.]]
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[[Category: Beck MR]]
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[[Category: Blaesing, F.]]
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[[Category: Blaesing F]]
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[[Category: Buhrdorf, R.]]
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[[Category: Buhrdorf R]]
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[[Category: Fischer, W.]]
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[[Category: Fischer W]]
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[[Category: Haas, R.]]
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[[Category: Haas R]]
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[[Category: Lanka, E.]]
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[[Category: Lanka E]]
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[[Category: Lurz, R.]]
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[[Category: Lurz R]]
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[[Category: Savvides, S N.]]
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[[Category: Savvides SN]]
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[[Category: Waksman, G.]]
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[[Category: Waksman G]]
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[[Category: Yeo, H J.]]
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[[Category: Yeo HJ]]
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[[Category: Bacterial type iv secretion]]
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[[Category: Helicobacter pylori]]
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[[Category: Hp0525]]
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[[Category: Sulfate]]
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[[Category: Virb11 atpase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:08:29 2008''
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Current revision

Crystal structure of the traffic ATPase (HP0525) of the Helicobacter pylori type IV secretion system bound by sulfate

PDB ID 1opx

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