1orc

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[[Image:1orc.gif|left|200px]]
 
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==CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]==
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The line below this paragraph, containing "STRUCTURE_1orc", creates the "Structure Box" on the page.
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<StructureSection load='1orc' size='340' side='right'caption='[[1orc]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1orc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ORC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1orc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1orc OCA], [https://pdbe.org/1orc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1orc RCSB], [https://www.ebi.ac.uk/pdbsum/1orc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1orc ProSAT]</span></td></tr>
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{{STRUCTURE_1orc| PDB=1orc | SCENE= }}
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</table>
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== Function ==
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'''CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]'''
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[https://www.uniprot.org/uniprot/RCRO_LAMBD RCRO_LAMBD] Cro represses genes normally expressed in early phage development and is necessary for the late stage of lytic growth. It does this by binding to the OL and OR operators regions normally used by the repressor protein for lysogenic maintenance.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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A rationally designed, genetically engineered, monomeric form of the Cro protein from bacteriophage lambda has been crystallized and its structure determined by isomorphous replacement and refined to a resolution of 1.54 A. The structure confirms the rationale of the design but, at the same time, reveals 1-2 A shifts throughout the monomer structure relative to the previously determined structure of the dimeric wild-type protein. These changes include a 1.6 A main-chain shift in part of the beta-sheet region of the molecule relative to the alpha-helical region and a 1.1 A shift of a buried phenylalanine within the core as well as a correlated 2.2 A shift in a solvent-exposed beta-hairpin. The conformational adjustments appear to reflect an inherent flexibility of the protein that is associated with its DNA-binding function.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/or/1orc_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1ORC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ORC OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1orc ConSurf].
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High-resolution structure of an engineered Cro monomer shows changes in conformation relative to the native dimer., Albright RA, Mossing MC, Matthews BW, Biochemistry. 1996 Jan 23;35(3):735-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8547253 8547253]
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<div style="clear:both"></div>
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[[Category: Enterobacteria phage lambda]]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Albright, R A.]]
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[[Category: Escherichia virus Lambda]]
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[[Category: Matthews, B W.]]
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[[Category: Large Structures]]
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[[Category: Mossing, M C.]]
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[[Category: Albright RA]]
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[[Category: Gene regulating protein]]
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[[Category: Matthews BW]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:11:16 2008''
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[[Category: Mossing MC]]

Current revision

CRO REPRESSOR INSERTION MUTANT K56-[DGEVK]

PDB ID 1orc

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