8rcg
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==W-formate dehydrogenase M405S from Desulfovibrio vulgaris== | |
| + | <StructureSection load='8rcg' size='340' side='right'caption='[[8rcg]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8rcg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris_str._Hildenborough Desulfovibrio vulgaris str. Hildenborough]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8RCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8RCG FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.005Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=H2S:HYDROSULFURIC+ACID'>H2S</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=W:TUNGSTEN+ION'>W</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8rcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8rcg OCA], [https://pdbe.org/8rcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8rcg RCSB], [https://www.ebi.ac.uk/pdbsum/8rcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8rcg ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Molybdenum- or tungsten-dependent formate dehydrogenases have emerged as significant catalysts for the chemical reduction of CO(2) to formate, with biotechnological applications envisaged in climate-change mitigation. The role of Met405 in the active site of Desulfovibrio vulgaris formate dehydrogenase AB (DvFdhAB) has remained elusive. However, its proximity to the metal site and the conformational change that it undergoes between the resting and active forms suggests a functional role. In this work, the M405S variant was engineered, which allowed the active-site geometry in the absence of methionine S(delta) interactions with the metal site to be revealed and the role of Met405 in catalysis to be probed. This variant displayed reduced activity in both formate oxidation and CO(2) reduction, together with an increased sensitivity to oxygen inactivation. | ||
| - | + | Structural and biochemical characterization of the M405S variant of Desulfovibrio vulgaris formate dehydrogenase.,Vilela-Alves G, Rebelo Manuel R, Pedrosa N, Cardoso Pereira IA, Romao MJ, Mota C Acta Crystallogr F Struct Biol Commun. 2024 May 1;80(Pt 5):98-106. doi: , 10.1107/S2053230X24003911. Epub 2024 May 1. PMID:38699971<ref>PMID:38699971</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8rcg" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Desulfovibrio vulgaris str. Hildenborough]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Manuel RR]] | ||
| + | [[Category: Mota C]] | ||
| + | [[Category: Pereira IC]] | ||
| + | [[Category: Romao MJ]] | ||
| + | [[Category: Vilela-Alves G]] | ||
Current revision
W-formate dehydrogenase M405S from Desulfovibrio vulgaris
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