1otc

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[[Image:1otc.gif|left|200px]]
 
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==THE O. NOVA TELOMERE END BINDING PROTEIN COMPLEXED WITH SINGLE STRAND DNA==
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The line below this paragraph, containing "STRUCTURE_1otc", creates the "Structure Box" on the page.
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<StructureSection load='1otc' size='340' side='right'caption='[[1otc]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1otc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OTC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1otc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1otc OCA], [https://pdbe.org/1otc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1otc RCSB], [https://www.ebi.ac.uk/pdbsum/1otc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1otc ProSAT]</span></td></tr>
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{{STRUCTURE_1otc| PDB=1otc | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TEBA_STENO TEBA_STENO] May function as protective capping of the single-stranded telomeric overhang. May also participate in telomere length regulation during DNA replication. Binds specifically to the T4G4-containing extension on the 3'strand and protects this region of the telomere from nuclease digestion and chemical modification.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ot/1otc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1otc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Telomeres are specialized protein-DNA complexes that compose the ends of eukaryotic chromosomes. Telomeres protect chromosome termini from degradation and recombination and act together with telomerase to ensure complete genome replication. We have determined the crystal structure of the two-subunit Oxytricha nova telomere end binding protein (OnTEBP) complexed with single strand telomeric DNA at 2.8 A resolution. The structure reveals four oligonucleotide/oligosaccharide-binding folds, three of which form a deep cleft that binds the ssDNA, and a fourth that forms an unusual protein-protein interaction between the alpha and beta subunits. This structure provides a molecular description of how the two subunits of OnTEBP recognize and bind ssDNA to form a sequence-specific, telomeric nucleoprotein complex that caps the very 3' ends of chromosomes.
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'''THE O. NOVA TELOMERE END BINDING PROTEIN COMPLEXED WITH SINGLE STRAND DNA'''
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Crystal structure of the Oxytricha nova telomere end binding protein complexed with single strand DNA.,Horvath MP, Schweiker VL, Bevilacqua JM, Ruggles JA, Schultz SC Cell. 1998 Dec 23;95(7):963-74. PMID:9875850<ref>PMID:9875850</ref>
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==Overview==
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Telomeres are specialized protein-DNA complexes that compose the ends of eukaryotic chromosomes. Telomeres protect chromosome termini from degradation and recombination and act together with telomerase to ensure complete genome replication. We have determined the crystal structure of the two-subunit Oxytricha nova telomere end binding protein (OnTEBP) complexed with single strand telomeric DNA at 2.8 A resolution. The structure reveals four oligonucleotide/oligosaccharide-binding folds, three of which form a deep cleft that binds the ssDNA, and a fourth that forms an unusual protein-protein interaction between the alpha and beta subunits. This structure provides a molecular description of how the two subunits of OnTEBP recognize and bind ssDNA to form a sequence-specific, telomeric nucleoprotein complex that caps the very 3' ends of chromosomes.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1OTC is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OTC OCA].
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</div>
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<div class="pdbe-citations 1otc" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Crystal structure of the Oxytricha nova telomere end binding protein complexed with single strand DNA., Horvath MP, Schweiker VL, Bevilacqua JM, Ruggles JA, Schultz SC, Cell. 1998 Dec 23;95(7):963-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9875850 9875850]
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*[[End-binding protein|End-binding protein]]
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[[Category: Protein complex]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Sterkiella nova]]
[[Category: Sterkiella nova]]
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[[Category: Bevilacqua, J M.]]
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[[Category: Bevilacqua JM]]
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[[Category: Horvath, M P.]]
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[[Category: Horvath MP]]
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[[Category: Ruggles, J A.]]
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[[Category: Ruggles JA]]
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[[Category: Schultz, S C.]]
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[[Category: Schultz SC]]
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[[Category: Schweiker, V L.]]
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[[Category: Schweiker VL]]
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[[Category: Ob fold]]
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[[Category: Oligonucleotide and oligosaccharide binding fold]]
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[[Category: Protein dna interaction]]
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[[Category: Protein protein interaction]]
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[[Category: Single strand dna binding protein]]
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[[Category: Telomere]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:15:35 2008''
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Current revision

THE O. NOVA TELOMERE END BINDING PROTEIN COMPLEXED WITH SINGLE STRAND DNA

PDB ID 1otc

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