1qfk

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(New page: 200px<br /> <applet load="1qfk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qfk, resolution 2.800&Aring;" /> '''STRUCTURE OF HUMAN...)
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[[Image:1qfk.gif|left|200px]]<br />
 
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<applet load="1qfk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qfk, resolution 2.800&Aring;" />
 
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'''STRUCTURE OF HUMAN FACTOR VIIA AND ITS IMPLICATIONS FOR THE TRIGGERING OF BLOOD COAGULATION'''<br />
 
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==Overview==
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==STRUCTURE OF HUMAN FACTOR VIIA AND ITS IMPLICATIONS FOR THE TRIGGERING OF BLOOD COAGULATION==
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Factor VIIa (EC 3.4.21.21) is a trypsin-like serine protease that plays a, key role in the blood coagulation cascade. On injury, factor VIIa forms a, complex with its allosteric regulator, tissue factor, and initiates blood, clotting. Although the structure of the binary complex has already been, determined [Banner, D. W., D'Arcy, A., Chene, C., Winkler, F. K., Guha, A., Konigsberg, W. H., Nemerson, Y. &amp; Kirchhofer, D. (1996) Nature, (London) 380, 41-46], the conformational effects of cofactor binding to, factor VIIa are not known in detail because of a lack of structural, information on free factor VIIa. Here we report the structure of, gamma-carboxyglutamic acid-domainless human coagulation factor VIIa at a, resolution of 2.8 A. The molecule adopts an extended conformation within, the crystal similar to that previously observed for the full-length, protein in complex with tissue factor. Detailed comparison of free and, tissue factor-bound factor VIIa reveals several structural differences., The binding mode of the active-site inhibitor D-Phe-Phe-Arg methyl ketone, differs in the two structures, suggesting a role for the cofactor in, substrate recognition. More importantly, a surface-exposed alpha-helix in, the protease domain (residues 307-312), which is located at the cofactor, recognition site, is distorted in the free form of factor VIIa. This, subtle structural difference sheds light on the mechanism of the dramatic, tissue factor-induced enhancement of factor VIIa activity.
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<StructureSection load='1qfk' size='340' side='right'caption='[[1qfk]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qfk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QFK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QFK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0Z6:D-PHENYLALANYL-N-[(2S,3S)-6-{[AMINO(IMINIO)METHYL]AMINO}-1-CHLORO-2-HYDROXYHEXAN-3-YL]-L-PHENYLALANINAMIDE'>0Z6</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=FUL:BETA-L-FUCOSE'>FUL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qfk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qfk OCA], [https://pdbe.org/1qfk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qfk RCSB], [https://www.ebi.ac.uk/pdbsum/1qfk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qfk ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/FA7_HUMAN FA7_HUMAN] Defects in F7 are the cause of factor VII deficiency (FA7D) [MIM:[https://omim.org/entry/227500 227500]. A hemorrhagic disease with variable presentation. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or repeated hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Finally, numerous subjects are completely asymptomatic despite very low factor VII levels.<ref>PMID:8043443</ref> <ref>PMID:2070047</ref> <ref>PMID:1634227</ref> <ref>PMID:8364544</ref> <ref>PMID:8204879</ref> <ref>PMID:7981691</ref> <ref>PMID:7974346</ref> <ref>PMID:8652821</ref> <ref>PMID:8844208</ref> <ref>PMID:8940045</ref> <ref>PMID:8883260</ref> <ref>PMID:9414278</ref> <ref>PMID:9576180</ref> <ref>PMID:9452082</ref> <ref>PMID:11091194</ref> <ref>PMID:11129332</ref> <ref>PMID:10862079</ref> <ref>PMID:12472587</ref> <ref>PMID:14717781</ref> <ref>PMID:19751712</ref> <ref>PMID:18976247</ref> <ref>PMID:19432927</ref> <ref>PMID:21206266</ref> <ref>PMID:21372693</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/FA7_HUMAN FA7_HUMAN] Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qf/1qfk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qfk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Factor VIIa (EC 3.4.21.21) is a trypsin-like serine protease that plays a key role in the blood coagulation cascade. On injury, factor VIIa forms a complex with its allosteric regulator, tissue factor, and initiates blood clotting. Although the structure of the binary complex has already been determined [Banner, D. W., D'Arcy, A., Chene, C., Winkler, F. K., Guha, A., Konigsberg, W. H., Nemerson, Y. &amp; Kirchhofer, D. (1996) Nature (London) 380, 41-46], the conformational effects of cofactor binding to factor VIIa are not known in detail because of a lack of structural information on free factor VIIa. Here we report the structure of gamma-carboxyglutamic acid-domainless human coagulation factor VIIa at a resolution of 2.8 A. The molecule adopts an extended conformation within the crystal similar to that previously observed for the full-length protein in complex with tissue factor. Detailed comparison of free and tissue factor-bound factor VIIa reveals several structural differences. The binding mode of the active-site inhibitor D-Phe-Phe-Arg methyl ketone differs in the two structures, suggesting a role for the cofactor in substrate recognition. More importantly, a surface-exposed alpha-helix in the protease domain (residues 307-312), which is located at the cofactor recognition site, is distorted in the free form of factor VIIa. This subtle structural difference sheds light on the mechanism of the dramatic tissue factor-induced enhancement of factor VIIa activity.
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==Disease==
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Structure of human factor VIIa and its implications for the triggering of blood coagulation.,Pike AC, Brzozowski AM, Roberts SM, Olsen OH, Persson E Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):8925-30. PMID:10430872<ref>PMID:10430872</ref>
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Known diseases associated with this structure: Factor VII deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=227500 227500]], Myocardial infarction, decreased susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=227500 227500]]
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1QFK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with GLC, FUC, CA and CH2 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Coagulation_factor_VIIa Coagulation factor VIIa], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.21 3.4.21.21] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QFK OCA].
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</div>
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<div class="pdbe-citations 1qfk" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structure of human factor VIIa and its implications for the triggering of blood coagulation., Pike AC, Brzozowski AM, Roberts SM, Olsen OH, Persson E, Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):8925-30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10430872 10430872]
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*[[Factor VIIa 3D structures|Factor VIIa 3D structures]]
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[[Category: Coagulation factor VIIa]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Brzozowski, A.M.]]
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[[Category: Brzozowski AM]]
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[[Category: Olsen, O.H.]]
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[[Category: Olsen OH]]
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[[Category: Persson, E.]]
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[[Category: Persson E]]
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[[Category: Pike, A.C.W.]]
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[[Category: Pike ACW]]
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[[Category: Roberts, S.M.]]
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[[Category: Roberts SM]]
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[[Category: CA]]
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[[Category: CH2]]
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[[Category: FUC]]
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[[Category: GLC]]
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[[Category: blood coagulation]]
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[[Category: serine protease]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 18:52:42 2007''
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Current revision

STRUCTURE OF HUMAN FACTOR VIIA AND ITS IMPLICATIONS FOR THE TRIGGERING OF BLOOD COAGULATION

PDB ID 1qfk

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