1p0h

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[[Image:1p0h.jpg|left|200px]]
 
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==Crystal Structure of Rv0819 from Mycobacterium Tuberculosis MshD-Mycothiol Synthase Coenzyme A Complex==
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The line below this paragraph, containing "STRUCTURE_1p0h", creates the "Structure Box" on the page.
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<StructureSection load='1p0h' size='340' side='right'caption='[[1p0h]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1p0h]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P0H FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
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{{STRUCTURE_1p0h| PDB=1p0h | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p0h OCA], [https://pdbe.org/1p0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p0h RCSB], [https://www.ebi.ac.uk/pdbsum/1p0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p0h ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of Rv0819 from Mycobacterium Tuberculosis MshD-Mycothiol Synthase Coenzyme A Complex'''
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== Function ==
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[https://www.uniprot.org/uniprot/MSHD_MYCTU MSHD_MYCTU] Catalyzes the transfer of acetyl from acetyl-CoA to desacetylmycothiol (Cys-GlcN-Ins) to form mycothiol.<ref>PMID:12375100</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Mycothiol is the predominant low-molecular weight thiol produced by actinomycetes, including Mycobacterium tuberculosis. The last reaction in the biosynthetic pathway for mycothiol is catalyzed by mycothiol synthase (MshD), which acetylates the cysteinyl amine of cysteine-glucosamine-inositol (Cys-GlcN-Ins). The crystal structure of MshD was determined in the presence of coenzyme A and acetyl-CoA. MshD consists of two tandem-repeated domains, each exhibiting the Gcn5-related N-acetyltransferase (GNAT) fold. These two domains superimpose with a root-mean-square deviation of 1.7 A over 88 residues, and each was found to bind one molecule of coenzyme, although the binding sites are quite different. The C-terminal domain has a similar active site to many GNAT members in which the acetyl group of the coenzyme is presented to an open active site slot. However, acetyl-CoA bound to the N-terminal domain is buried, and is apparently not positioned to promote acetyl transfer. A modeled substrate complex indicates that Cys-GlcN-Ins would only fill a portion of a negatively charged channel located between the two domains. This is the first structure determined for an enzyme involved in the biosynthesis of mycothiol.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p0/1p0h_consurf.spt"</scriptWhenChecked>
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1P0H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P0H OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of mycothiol synthase (Rv0819) from Mycobacterium tuberculosis shows structural homology to the GNAT family of N-acetyltransferases., Vetting MW, Roderick SL, Yu M, Blanchard JS, Protein Sci. 2003 Sep;12(9):1954-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12930994 12930994]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p0h ConSurf].
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[[Category: Mycobacterium tuberculosis h37rv]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Blanchard, J S.]]
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<references/>
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[[Category: Roderick, S L.]]
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__TOC__
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[[Category: Vetting, M W.]]
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</StructureSection>
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[[Category: Yu, M.]]
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[[Category: Large Structures]]
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[[Category: Acetyltransferase]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Coenzyme a complex]]
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[[Category: Blanchard JS]]
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[[Category: Gnat fold]]
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[[Category: Roderick SL]]
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[[Category: Mshd]]
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[[Category: Vetting MW]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:31:33 2008''
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[[Category: Yu M]]

Current revision

Crystal Structure of Rv0819 from Mycobacterium Tuberculosis MshD-Mycothiol Synthase Coenzyme A Complex

PDB ID 1p0h

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