8vci
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==SARS-CoV-2 Frameshift Stimulatory Element with Upstream Multibranch Loop== | |
| + | <StructureSection load='8vci' size='340' side='right'caption='[[8vci]], [[Resolution|resolution]] 6.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8vci]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8VCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8VCI FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.1Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8vci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8vci OCA], [https://pdbe.org/8vci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8vci RCSB], [https://www.ebi.ac.uk/pdbsum/8vci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8vci ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshift stimulatory element (FSE) is necessary for programmed -1 ribosomal frameshifting (-1 PRF) and optimized viral efficacy. The FSE has an abundance of context-dependent alternate conformations, but two of the structures most crucial to -1 PRF are an attenuator hairpin and a three-stem H-type pseudoknot structure. A crystal structure of the pseudoknot alone features three RNA stems in a helically stacked linear structure, whereas a 6.9 A cryo-EM structure including the upstream heptameric slippery site resulted in a bend between two stems. Our previous research alluded to an extended upstream multibranch loop that includes both the attenuator hairpin and the slippery site-a conformation not previously modeled. We aim to provide further context to the SARS-CoV-2 FSE via computational and medium resolution cryo-EM approaches, by presenting a 6.1 A cryo-EM structure featuring a linear pseudoknot structure and a dynamic upstream multibranch loop. | ||
| - | + | Structure of the SARS-CoV-2 Frameshift Stimulatory Element with an Upstream Multibranch Loop.,Peterson JM, Becker ST, O'Leary CA, Juneja P, Yang Y, Moss WN Biochemistry. 2024 May 21;63(10):1287-1296. doi: 10.1021/acs.biochem.3c00716. , Epub 2024 May 10. PMID:38727003<ref>PMID:38727003</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8vci" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Moss | + | __TOC__ |
| - | [[Category: O | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| + | [[Category: Severe acute respiratory syndrome coronavirus 2]] | ||
| + | [[Category: Becker ST]] | ||
| + | [[Category: Juneja P]] | ||
| + | [[Category: Moss WN]] | ||
| + | [[Category: O'Leary CA]] | ||
| + | [[Category: Peterson JM]] | ||
| + | [[Category: Yang Y]] | ||
Current revision
SARS-CoV-2 Frameshift Stimulatory Element with Upstream Multibranch Loop
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